State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China.
Org Biomol Chem. 2018 May 9;16(18):3353-3357. doi: 10.1039/c8ob00169c.
A new bench-stable reagent with double diazonium sites was designed and synthesized for protein crosslinking. Based on the highly efficient diazonium-Tyr coupling reaction, a direct mixture of the reagent and tobacco mosaic virus led to the formation of a new hydrogel, which could be degraded by chemicals and could be used to encapsulate small molecules for sustained release. Because plant viruses exhibit many chemical characteristics like protein labelling and nucleic acid packaging, the virus-based hydrogel will have large chemical space for further functionalization. Besides, this dual-diazonium reagent should be a generally useful crosslinker for chemical biology and biomaterials.
设计并合成了一种新型的、双重重氮位点的、在台面上稳定的试剂，用于蛋白质交联。基于高效的重氮-Tyr 偶联反应，将该试剂与烟草花叶病毒直接混合，可形成一种新的水凝胶，该水凝胶可被化学物质降解，并可用于封装小分子以实现持续释放。由于植物病毒表现出许多化学特性，如蛋白质标记和核酸包装，因此基于病毒的水凝胶将具有更大的化学空间用于进一步的功能化。此外，这种双重重氮试剂应该是用于化学生物学和生物材料的一种普遍有用的交联剂。
