开发用于烟草花叶病毒更快交联以形成水凝胶的改良双叠氮试剂。
Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels.
作者信息
Ma Dejun, Chen Zhuoyue, Yi Long, Xi Zhen
机构信息
State Key Laboratory of Elemento-Organic Chemistry, Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University Tianjin 300071 China
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology Beijing 100029 China
出版信息
RSC Adv. 2019 Sep 17;9(50):29070-29077. doi: 10.1039/c9ra05630k. eCollection 2019 Sep 13.
New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds DDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus-based hydrogels.
设计并合成了具有两个重氮位点的新型稳定试剂用于蛋白质交联。由于重氮-酪氨酸偶联反应更快，烟草花叶病毒与试剂DDA-3交联形成的水凝胶可在室温下1分钟内制备完成。此外，引入二硫键的水凝胶DDA-4可被二硫苏糖醇化学降解。我们的结果为直接构建基于病毒的水凝胶提供了一种简便方法。
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