Koroiva Ricardo, de Souza Mirian S, Roque Fabio de Oliveira, Pepinelli Mateus
Laboratório de Ecologia, InBio, Universidade Federal de Mato Grosso do Sul, Cidade Universitária, Campo Grande, MS, Brazil.
Programa de Pós-Graduação em Ecologia e Recursos Naturais, Universidade Federal de São Carlos, Rodovia Washington Luís, São Carlos, SP, Brazil.
J Med Entomol. 2018 Jun 28;55(4):1055-1061. doi: 10.1093/jme/tjy045.
Here, we analyze 248 DNA barcode sequences of 35 fly species of forensic importance in Brazil. DNA barcoding can be effectively used for specimen identification of these species, allowing the unambiguous identification of 31 species, an overall success rate of 88%. Our results show a high rate of success for molecular identification using DNA barcoding sequences and open new perspectives for immature species identification, a subject on which limited forensic investigations exist in Tropical regions. We also address the implications of building a robust forensic DNA barcode database. A geographic bias is recognized for the COI dataset available for forensically important fly species in Brazil, with concentration of sequences from specimens collected mainly in sites located in the Cerrado, Mata Atlântica, and Pampa biomes.
在此，我们分析了巴西35种具有法医重要性的蝇类物种的248个DNA条形码序列。DNA条形码可有效用于这些物种的标本鉴定，能够明确鉴定出31个物种，总体成功率为88%。我们的结果表明，使用DNA条形码序列进行分子鉴定的成功率很高，并为未成熟物种的鉴定开辟了新的前景，而在热带地区，关于这一主题的法医调查有限。我们还讨论了建立一个强大的法医DNA条形码数据库的意义。对于巴西具有法医重要性的蝇类物种的细胞色素氧化酶亚基I（COI）数据集，存在地理偏差，序列主要集中于从塞拉多、大西洋森林和潘帕生物群落的地点采集的标本。
