Luteibacter pinisoli sp. nov., a casein degrading bacterium isolated from rhizospheric soil of Pinus koraiensis.

A yellow pigmented, Gram-staining negative, motile and rod-shaped novel bacterial strain, designated MAH-14 was isolated from rhizospheric soil and was characterized using a polyphasic approach. The isolated strain was aerobic, oxidase and catalase were positive, optimum growth temperature and pH were 28-30 °C and 6.5, respectively. The novel strain is able to hydrolyze casein, starch, esculin, gelatin, L-tyrosine, DNA, tween 80, tween 20, L-arginine and 4-nitrophenyl-BD-galactopyranoside. On the basis of 16S rRNA gene sequence analysis, strain MAH-14 belongs to the genus Luteibacter and is most closely related to Luteibacter yeojuensis R2A16-10 (98.5%), Luteibacter anthropi CCUG 25036 (98.4%) and Luteibacter rhizovicinus LJ96 (98.3%). In DNA-DNA hybridization experiments, the DNA relatedness between strain MAH-14 and its closest phylogenetic neighbor was below 45.0%. The predominant respiratory quinone and the DNA G + C content of the novel strain were ubiquinone-8 and 63.5 mol%, respectively. The novel strain MAH-14 is able to produce flexirubin-type pigments. The major cellular fatty acids were C, summed feature 3 (C ω7c and/or C ω6c) and summed feature 9 (C ω9c and/or C 10-methyl). The DNA-DNA hybridization results and results of the genotypic analysis in combination with chemotaxonomic and physiological data revealed that strain MAH-14 represented a novel species within the genus Luteibacter, for which the name Luteibacter pinisoli, is proposed. The type strain is MAH-14 (= KACC 19298 = CGMCC 1.16227).