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新种,从富营养化池塘中分离得到及噬菌体vB_LflM-Pluto的分离与鉴定

sp. nov., Isolated from a Eutrophic Pond and Isolation and Characterization of Phage vB_LflM-Pluto.

作者信息

Friedrich Ines, Kuritsyn Alisa, Hertel Robert, Daniel Rolf

机构信息

Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University of Göttingen, 37077 Göttingen, Germany.

FG Synthetic Microbiology, Institute of Biotechnology, BTU Cottbus-Senftenberg, 01968 Senftenberg, Germany.

出版信息

Microorganisms. 2023 Jan 24;11(2):307. doi: 10.3390/microorganisms11020307.

DOI:10.3390/microorganisms11020307
PMID:36838271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9965599/
Abstract

is a genus of the family. The present study describes a novel species within the genus (EIF3). The strain was analyzed genomically, morphologically and physiologically. Average nucleotide identity analysis revealed that it is a new species of . In silico analysis indicated two putative prophages (one incomplete, one intact). EIF3 cells form an elliptical morphotype with an average length of 2.0 µm and width of 0.7 µm and multiple flagella at one end. The bacterial strain is an aerobic Gram-negative with optimal growth at 30 °C. EIF3 is resistant towards erythromycin, tetracycline and vancomycin. We propose the name sp. nov. with EIF3 (=DSM 112537 = LMG 32416) as type strain. Further, we describe the first known -associated bacteriophage called vB_LflM-Pluto.

摘要

是该科的一个属。本研究描述了该属(EIF3)内的一个新物种。对该菌株进行了基因组、形态和生理分析。平均核苷酸同一性分析表明它是 的一个新物种。电子分析表明有两个推定的原噬菌体(一个不完整,一个完整)。EIF3细胞形成椭圆形形态型,平均长度为2.0 µm,宽度为0.7 µm,一端有多个鞭毛。该细菌菌株是需氧革兰氏阴性菌,在30°C时生长最佳。EIF3对红霉素、四环素和万古霉素耐药。我们提议将 新种命名为 ,以EIF3(=DSM 112537 = LMG 32416)作为模式菌株。此外,我们描述了第一个已知的与 相关的噬菌体,称为vB_LflM-Pluto。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/b61930261d2e/microorganisms-11-00307-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/ccb6e790ead7/microorganisms-11-00307-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/c87f29cd5ff5/microorganisms-11-00307-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/dd766d3a524f/microorganisms-11-00307-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/b61930261d2e/microorganisms-11-00307-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/ccb6e790ead7/microorganisms-11-00307-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/c87f29cd5ff5/microorganisms-11-00307-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/dd766d3a524f/microorganisms-11-00307-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/9965599/b61930261d2e/microorganisms-11-00307-g004.jpg

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