1 Department of Life Science and Graduate Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, Republic of China.
2 Department of Pathology, Buddhist Tzu Chi General Hospital, Tzu Chi University, Hualien, Taiwan, Republic of China.
Cell Transplant. 2018 Feb;27(2):256-263. doi: 10.1177/0963689717741139.
In this study, we explored the effect of the water extract of Cinnamomum osmophloeum Kanehira (COK) leaves on hair growth by in vitro and in vivo assays. Using an in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, it was found that the proliferation of rat vibrissae and human hair dermal papilla cells (hDPCs) was significantly enhanced by the COK leaf extract treatment. As determined by quantitative real-time polymerase chain reaction (RT-PCR), the messenger RNA (mRNA) levels of some hair growth-related factors including vascular endothelial growth factor, keratinocyte growth factor (KGF), and transforming growth factor-β2 were found to be higher in the cultured hDPCs exposed to COK leaf extract than those in the untreated control group. In the hair-depilated C57BL/6 mouse model, the stimulation of hair growth was demonstrated in the group of COK leaf extract treatment. Both photographical and histological observations revealed the promotion of the anagen phase in the hair growth cycle by the COK leaf extract in the C57BL/6 mice. Finally, the ultra performance liquid chromatography (UPLC) showed that the COK extract contained mostly cinnamic aldehyde and a small amount of cinnamic acid. The results suggest that the COK leaf extract may find use for the treatment of hair loss.
在这项研究中，我们通过体外和体内实验探索了肉桂（Cinnamomum osmophloeum Kanehira，COK）叶水提取物对毛发生长的影响。通过体外 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测发现，COK 叶提取物处理显著促进了大鼠触须和人头皮毛乳头细胞（hDPC）的增殖。通过定量实时聚合酶链反应（RT-PCR）测定，与未经处理的对照组相比，暴露于 COK 叶提取物的培养 hDPC 中一些与毛发生长相关的因子（包括血管内皮生长因子、角质细胞生长因子（KGF）和转化生长因子-β2）的信使 RNA（mRNA）水平更高。在 C57BL/6 无毛小鼠模型中，COK 叶提取物处理组的毛发再生得到了证实。在 C57BL/6 小鼠中，COK 叶提取物通过促进毛发生长周期中的生长期，无论是通过照片还是组织学观察都得到了证实。最后，超高效液相色谱（UPLC）表明，COK 提取物主要含有肉桂醛，少量肉桂酸。这些结果表明，COK 叶提取物可能可用于脱发的治疗。
