Park Hyeon Ju, Jin Guang-Ri, Jung Jae Hyun, Hwang Su Bin, Lee Su Hyun, Lee Bog-Hieu
Department of Food and Nutrition, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
Evid Based Complement Alternat Med. 2021 Mar 13;2021:6661373. doi: 10.1155/2021/6661373. eCollection 2021.
This study investigated the hair regeneration promotion and hair loss prevention properties of Nelumbinis Semen (NS) extract and . The effect of NS on the proliferation and migration of human dermal papilla cells (hDPCs) was measured via CCK-8 and scratch migration assays, after which the antioxidant activity of NS was also quantified. NS extracts were then applied to the back of 7-week-old C57BL/6 mice for 3 weeks to monitor hair growth patterns and hair follicle (HF) histology. The mice were divided into three groups: negative control group (NC; DMSO), positive control group (PC; 3% minoxidil), and experimental group (NS extract 1,000 ppm). Moreover, to study the molecular mechanisms by which NS extract regenerates hair growth, real-time PCR was used to analyze factors related to the hair growth cycle. The NS extracts were found to possess high antioxidant properties due to their high flavonoid contents and electron-donating ability. Moreover, NS extracts enhanced hDPC proliferation and migration in a concentration-dependent manner (15.63-125 ppm). The hair growth index and growth area of the NS group (2.81 score, 81%) on day 14 were higher than those of the PC group (2.65 score, 68%) ( < 0.05). Additionally, the HFs of the NS group were located deep in the subcutis, similar to the PC group with developed hair roots. Moreover, the mRNA expression of VEGF and IGF-1 was higher in the NS group compared to the PC group, whereas TGF-1 expression was lower ( < 0.05). Our findings indicate that NS modulates hair growth by increasing IGF-1 and VEGF expression while inhibiting that of TGF-1. Therefore, our findings suggest that NS extract is a promising new hair loss treatment derived from a natural substance that helps promote hair growth and prevent hair loss.
本研究调查了莲子提取物促进毛发生长和预防脱发的特性。通过CCK-8和划痕迁移试验测定了莲子提取物对人毛乳头细胞（hDPCs）增殖和迁移的影响，之后还对莲子提取物的抗氧化活性进行了定量分析。然后将莲子提取物应用于7周龄C57BL/6小鼠的背部3周，以监测毛发生长模式和毛囊组织学变化。小鼠被分为三组：阴性对照组（NC；二甲基亚砜）、阳性对照组（PC；3%米诺地尔）和实验组（1000 ppm莲子提取物）。此外，为了研究莲子提取物促进毛发生长的分子机制，采用实时定量PCR分析与毛发生长周期相关的因子。发现莲子提取物由于其高黄酮含量和供电子能力而具有高抗氧化特性。此外，莲子提取物以浓度依赖的方式（15.63 - 125 ppm）增强hDPC的增殖和迁移。第14天，莲子组的毛发生长指数和生长面积（2.81分，81%）高于阳性对照组（2.65分，68%）（P < 0.05）。此外，莲子组的毛囊位于皮下深处，与阳性对照组相似，发根发达。而且，与阳性对照组相比，莲子组中血管内皮生长因子（VEGF）和胰岛素样生长因子-1（IGF-1）的mRNA表达较高，而转化生长因子-1（TGF-1）的表达较低（P < 0.05）。我们的研究结果表明，莲子提取物通过增加IGF-1和VEGF的表达同时抑制TGF-1的表达来调节毛发生长。因此，我们的研究结果表明，莲子提取物是一种有前景的新型脱发治疗药物，它源自一种有助于促进毛发生长和预防脱发的天然物质。
