Weber Mareike, Schünemann Wiebke, Fuß Janina, Kämpfer Peter, Lipski André
Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Ernährungs- und Lebensmittelwissenschaften, Abteilung Lebensmittelmikrobiologie und -hygiene, Meckenheimer Allee 168, 53115 Bonn, Germany.
Max Planck-Genome-Centre Cologne, Carl-von-Linné-Weg 10, 50829 Köln, Germany.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1830-1838. doi: 10.1099/ijsem.0.002732. Epub 2018 Apr 11.
Five Gram-stain-negative, rod-shaped, none-spore-forming isolates were obtained from biofilms on different sites of a milking machine in Germany. Another strain with similar morphological characteristics was isolated from dirty dishes. Based on phylogenetic analysis of the 16S rRNA and gyrB genes, all isolates were assigned to the genus Stenotrophomonas, but were divided into three different groups. Chemotaxonomic characterization of the isolates led to the detection of iso-C15 : 0 and anteiso-C15 : 0 as the predominant cellular fatty acids, as well as small amounts of the hydroxyl fatty acids iso-C11 : 0 3-OH, C12 : 0 3-OH and iso-C13 : 0 3-OH. One group could be assigned to the species Stenotrophomonas maltophilia, while the genome sequences of two groups displayed average nucleotide identity values of less than 94 % between each other and the genome sequences of the next related type strains Stenotrophomonas maltophilia ATCC 13637 and Stenotrophomonas rhizophila DSM 14405. Further phylogenetic, phenotypic and chemotaxonomic analyses enabled the differentiation of these strains from these closely related species. They are therefore considered to represent two novel species, for which the names Stenotrophomonaslactitubi and Stenotrophomonasindicatrix are proposed, with strains M15 (=DSM 104152=LMG29943) and WS40 (=DSM28278=LMG29942) as type strains.
从德国一台挤奶机不同部位的生物膜中获得了5株革兰氏阴性、杆状、无芽孢形成的分离株。从脏盘子中分离出另一株具有相似形态特征的菌株。基于16S rRNA和gyrB基因的系统发育分析，所有分离株均归为嗜麦芽窄食单胞菌属，但分为三个不同的组。对分离株的化学分类学特征进行分析后，检测到异-C15 : 0和anteiso-C15 : 0为主要的细胞脂肪酸，以及少量的羟基脂肪酸异-C11 : 0 3-OH、C12 : 0 3-OH和异-C13 : 0 3-OH。其中一组可归为嗜麦芽窄食单胞菌物种，但另外两组的基因组序列彼此之间以及与下一个相关模式菌株嗜麦芽窄食单胞菌ATCC 13637和嗜根窄食单胞菌DSM 14405的基因组序列之间的平均核苷酸同一性值均小于94 %。进一步的系统发育、表型和化学分类学分析能够将这些菌株与这些密切相关的物种区分开来。因此，它们被认为代表两个新物种，为此提出了嗜麦芽窄食单胞菌乳酸管菌和嗜麦芽窄食单胞菌指示菌这两个名称，菌株M15（=DSM 104152=LMG29943）和WS40（=DSM28278=LMG29942）为模式菌株。
