The Key Laboratory of National Forestry and Grassland Administration on Forest Protection, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China.
Antonie Van Leeuwenhoek. 2020 Oct;113(10):1447-1454. doi: 10.1007/s10482-020-01453-y. Epub 2020 Aug 3.
A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15 (99.6%), Stenotrophomonas indicatrix WS40 (99.4%), Stenotrophomonas maltophilia IAM 12423 (99.2%) and Stenotrophomonas pavanii LMG 25348 (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15, S. indicatrix WS40, S. maltophilia IAM 12423, S. pavanii LMG 25348, and Pseudomonas geniculata ATCC 19374 were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the genus Stenotrophomonas. The DNA G + C content of TPQG1-4 is 67.1 mol%. The major fatty acids were iso-C (25.4%) and anteiso-C (17.0%). The polar lipids of TPQG1-4 included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas, for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4 (= CFCC 15341 = LMG 31208).
一株革兰氏阴性、兼性厌氧、运动的细菌菌株 TPQG1-4 是从具有斑点病的岗柃叶片中分离得到的。该分离株采用多相分类方法进行了研究。16S rRNA 基因测序和分析表明，该新菌株与韧皮养菌 M15（99.6%）、印度色杆菌 WS40（99.4%）、嗜麦芽寡养单胞菌 IAM 12423（99.2%）和产碱假单胞菌 LMG 25348（99.0%）的序列相似度最高。在基于 16S rRNA 基因序列的系统发育树中，该新菌株与其他韧皮养菌属的物种独立分支。新型分离株与 S. lactitubi M15、S. indicatrix WS40、S. maltophilia IAM 12423、S. pavanii LMG 25348 和 Pseudomonas geniculata ATCC 19374 之间的平均核苷酸同一性值分别为 87.2%、87.3%、86.3%、88.0%和 81.3%，表明该分离株是一种新的韧皮养菌属物种。TPQG1-4 的 DNA G+C 含量为 67.1mol%。主要脂肪酸为异-C（25.4%）和anteiso-C（17.0%）。TPQG1-4 的极性脂包括磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、氨基磷脂和磷脂。基于表型和基因型特征，该菌株代表了韧皮养菌属中的一个新种，建议将其命名为 Cyclobalanopsidis 韧皮养菌 sp. nov. 。模式菌株为 TPQG1-4（=CFCC 15341=LMG 31208）。
