Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Glheung-gu, Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1522-1526. doi: 10.1099/ijs.0.014662-0. Epub 2009 Aug 14.
A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01(T), was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01(T) belonged to the Gammaproteobacteria and was most closely related to Stenotrophomonas koreensis KCTC 12211(T) (98.4 % similarity), Stenotrophomonas humi R-32729(T) (97.2 %), Stenotrophomonas terrae R-32768 (97.1 %), Stenotrophomonas maltophilia DSM 50170(T) (96.9 %) and Stenotrophomonas nitritireducens DSM 12575(T) (96.8 %). Chemotaxonomic analyses revealed that strain DCY01(T) possessed a quinone system with Q-8 as the predominant compound, and iso-C(15 : 0) (28.2 %), C(16 : 0) 10-methyl (13.2 %), iso-C(15 : 1) F (10.8 %) and C(15 : 0) (7.5 %) as major fatty acids, corroborating assignment of strain DCY01(T) to the genus Stenotrophomonas. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01(T) represents a species distinct from recognized Stenotrophomonas species. Based on these data, DCY01(T) (=KCTC 12539(T)=NBRC 101154(T)) should be classified as the type strain of a novel species of the genus Stenotrophomonas, for which the name Stenotrophomonas ginsengisoli sp. nov. is proposed.
一株革兰氏阴性、非芽孢形成、杆状细菌，被命名为 DCY01(T) 菌株，从韩国人参田土壤中分离得到，并对其进行了特征描述，以确定其分类地位。16S rRNA 基因序列分析表明，菌株 DCY01(T) 属于γ变形菌纲，与 Stenotrophomonas koreensis KCTC 12211(T)（相似度 98.4%）、Stenotrophomonas humi R-32729(T)（相似度 97.2%）、Stenotrophomonas terrae R-32768（相似度 97.1%）、Stenotrophomonas maltophilia DSM 50170(T)（相似度 96.9%）和 Stenotrophomonas nitritireducens DSM 12575(T)（相似度 96.8%）最为密切相关。化学分类分析表明，菌株 DCY01(T) 具有以 Q-8 为主要成分的醌系统，主要脂肪酸为 iso-C(15:0)（28.2%）、C(16:0) 10-甲基（13.2%）、iso-C(15:1) F（10.8%）和 C(15:0)（7.5%），这与将菌株 DCY01(T) 归入 Stenotrophomonas 属的分类学归属相符。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。DNA-DNA 杂交和生理生化试验结果清楚地表明，菌株 DCY01(T) 代表了一个与已鉴定的 Stenotrophomonas 种不同的种。基于这些数据，DCY01(T)（=KCTC 12539(T)=NBRC 101154(T)）应被归类为 Stenotrophomonas 属的一个新种的模式株，建议将其命名为人参土壤 Stenotrophomonas ginsengisoli sp. nov.。
