Lin Yang, Ye Shandong, He Yuanyuan, Li Sumei, Chen Yan, Zhai Zhimin
School of Medicine, Shandong University, Jinan, Shandong 250100, China.
Department of Pediatrics, Anhui Provincial Hospital, Hefei, Anhui 230001, China.
Arch Endocrinol Metab. 2018 Apr 5;62(2):212-220. doi: 10.20945/2359-3997000000029. Print 2018 Mar-Apr.
To observe the effect of short-term insulin intensive treatment on the monocyte chemoattractant protein-1 (MCP-1) as well as on the nuclear factor-kappa B (NF-κB) expression of peripheral blood monocyte. This is also in addition to observing the serum MCP-1 level in newlydiagnosed type 2 diabetic patients and probing its anti-inflammation effects.
Twenty newly-diagnosed type 2 diabetic patients were treated with an insulin intensive treatment for 2 weeks. MCP-1 and NF-κB expression on the monocyte surface were measured with flow cytometry, the serum MCP-1 level was measured by enzyme linked immunosorbent assay (ELISA) during pretreatment and post-treatment.
After 2 weeks of the treatment, MCP-1 and NF-κB protein expression of peripheral blood monocyte and serum MCP-1 levels decreased significantly compared with those of pre-treatment, which were (0.50 ± 0.18)% vs (0.89 ± 0.26)% (12.22 ± 2.80)% vs (15.53 ± 2.49)% and (44.53 ± 3.97) pg/mL vs (49.53 ± 3.47) pg/mL, respectively (P < 0.01). The MCP-1 expression on monocyte surface had a significant positive relationship with serum MCP-1 levels (r = 0.47, P < 0.01).
Short-term insulin intensive therapy plays a role in alleviating the increased inflammation reaction in type 2 diabetics.
观察短期胰岛素强化治疗对单核细胞趋化蛋白-1（MCP-1）以及外周血单核细胞核因子-κB（NF-κB）表达的影响。此外，观察新诊断2型糖尿病患者血清MCP-1水平，并探讨其抗炎作用。
20例新诊断的2型糖尿病患者接受胰岛素强化治疗2周。采用流式细胞术检测单核细胞表面MCP-1和NF-κB表达，治疗前及治疗后采用酶联免疫吸附测定（ELISA）法检测血清MCP-1水平。
治疗2周后，外周血单核细胞MCP-1和NF-κB蛋白表达及血清MCP-1水平较治疗前显著降低，分别为（0.50±0.18）%对（0.89±0.26）%、（12.22±2.80）%对（15.53±2.49）%、（44.53±3.97）pg/mL对（49.53±3.47）pg/mL（P<0.01）。单核细胞表面MCP-1表达与血清MCP-1水平呈显著正相关（r=0.47，P<0.01）。
短期胰岛素强化治疗对减轻2型糖尿病患者炎症反应增强有作用。
