Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Molecules. 2018 Apr 10;23(4):870. doi: 10.3390/molecules23040870.
Oxidative stress, which is caused by Amyloid-β deposition in brain, plays an important role in Alzheimer's disease. In this study, we found that lignans from rattan stems (rsSCH-L) could reduce the escape latency and the distance travelled by the Aβ injected rats while the crossing platform time was enhanced in the Morris water maze test. Further research demonstrated that lignans from rsSCH-L attenuated Aβ-induced neuronal cell injury by increasing the content of SOD and GSH-Px and decreasing the levels of LDH, ROS, and MDA. Moreover, rsSCH-L also inhibited the apoptosis of primary neuronal cells. The mechanisms of the apoptosis were related with the downregulation of caspase-3, caspase-8, Bax, and upregulation of Bcl-2. Taken together, the results show that rsSCH-L can improve cognitive ability in vivo. Meanwhile rsSCH-L exhibit a neuroprotective environment against oxidative stress and apoptosis in vitro. Therefore, rsSCH-L may be a potential therapeutic agent for this neurodegenerative disease.
氧化应激是由脑内淀粉样蛋白-β沉积引起的，在阿尔茨海默病中起重要作用。在这项研究中，我们发现来自藤茎的木脂素（rsSCH-L）可以减少 Aβ 注射大鼠的逃逸潜伏期和行进距离，同时在 Morris 水迷宫测试中增强穿越平台时间。进一步的研究表明，rsSCH-L 通过增加 SOD 和 GSH-Px 的含量，降低 LDH、ROS 和 MDA 的水平，减轻 Aβ 诱导的神经元细胞损伤。此外，rsSCH-L 还抑制原代神经元细胞的凋亡。凋亡的机制与下调 caspase-3、caspase-8、Bax 和上调 Bcl-2 有关。总之，这些结果表明 rsSCH-L 可以改善体内认知能力。同时，rsSCH-L 在体外表现出对氧化应激和细胞凋亡的神经保护作用。因此，rsSCH-L 可能是这种神经退行性疾病的潜在治疗剂。
