一种用于检测 SO₂ 的新型比色荧光探针及其在活细胞成像中的应用。
A Novel Colorimetric Fluorescent Probe for SO₂ and Its Application in Living Cells Imaging.
机构信息
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
出版信息
Molecules. 2018 Apr 10;23(4):871. doi: 10.3390/molecules23040871.
A novel chromenylium-based fluorescent probe was exploited for sulphur dioxide (SO₂) detecting. The probe displayed a remarkable fluorescence turn-on response towards SO₂ based on the nucleophilic addition reaction to the carbon-carbon double bond with 105 nm Stock shift. The probe was successfully applied for the quantification of SO₂.The linear detection range was from 0-160 μM with the detection limit as low as 99.27 nM. It also exhibited high selectivity for SO₂ than other reactive species and amino acids. Furthermore, cell staining experiments indicated that the probe was cell membrane permeable and could be used for high-performance imaging of SO₂ in living cells. The superior properties of the probe made it highly promising for use in chemical and biological applications.
一种新型基于色烯鎓的荧光探针被开发用于检测二氧化硫 (SO₂)。该探针基于对碳-碳双键的亲核加成反应，对 SO₂ 表现出显著的荧光开启响应，斯托克位移为 105nm。该探针成功用于 SO₂ 的定量检测。线性检测范围为 0-160μM，检测限低至 99.27 nM。它对 SO₂ 的选择性也高于其他反应性物质和氨基酸。此外，细胞染色实验表明，该探针具有细胞膜通透性，可用于活细胞中 SO₂ 的高分辨率成像。该探针具有优异的性能，有望在化学和生物应用中得到广泛应用。
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