Safaei Mehdi, Jafarpour Seyed Masoud, Mohseni Mehran, Salimian Morteza, Akbari Hossein, Karami Fateme, Aliasgharzadeh Akbar, Farhood Bagher
Department of Medical Physics and Radiology, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Department of Medical Laboratory, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Indian J Nucl Med. 2018 Jan-Mar;33(1):20-24. doi: 10.4103/ijnm.IJNM_89_17.
Iodine-131 is used as a radiopharmaceutical to treat thyroid cancer. The current study aimed to evaluate the effects of vitamins E and C on the level of DNA double-strand breaks (DSBs) caused by Radioiodine-131 (I-131) in human lymphocytes.
Whole blood samples from human volunteers were incubated with a certain concentration of vitamins. After 1-h incubation, the samples were incubated with 20 μCi I-131/2 mL (blood + NaCl) for 1 h. To evaluate the effects of antioxidants, lymphocytes were separated, and the mean DSBs/cell was measured for each sample through γ-H2AX assay.
After 1-h incubation with 20 μCi I-131/2 mL (blood + NaCl), iodine-131 increased the level of DSBs by 102.9%, compared with the background group. Vitamins E and C reduced the level of DSBs by 21.5% and 36.4%, respectively.
Using vitamins E and C as antioxidants can reduce the toxicity of I-131. Furthermore, vitamin C provided the more protection for DNA, compared with vitamin E.
碘-131用作放射性药物治疗甲状腺癌。本研究旨在评估维生素E和维生素C对131碘（I-131）所致人淋巴细胞DNA双链断裂（DSB）水平的影响。
将人类志愿者的全血样本与一定浓度的维生素一起孵育。孵育1小时后，将样本与20μCi I-131/2 mL（血液+氯化钠）孵育1小时。为评估抗氧化剂的作用，分离淋巴细胞，并通过γ-H2AX检测法测量每个样本的平均DSB/细胞数。
与背景组相比，与20μCi I-131/2 mL（血液+氯化钠）孵育1小时后，碘-131使DSB水平增加了102.9%。维生素E和维生素C分别使DSB水平降低了21.5%和36.4%。
使用维生素E和维生素C作为抗氧化剂可降低I-131的毒性。此外，与维生素E相比，维生素C对DNA的保护作用更强。
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