Department of Laboratory Medicine, Medical Microbiology, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3109-3119. doi: 10.1099/ijs.0.059600-0. Epub 2014 Jun 18.
We previously discovered a symbiotic lactic acid bacterial (LAB) microbiota in the honey stomach of the honeybee Apis mellifera. The microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. 16S rRNA gene sequence analyses and phenotypic and genetic characteristics revealed that the phylotypes isolated represent seven novel species. One grouped with Lactobacillus kunkeei and the others belong to the Lactobacillus buchneri and Lactobacillus delbrueckii subgroups of Lactobacillus. We propose the names Lactobacillus apinorum sp. nov., Lactobacillus mellifer sp. nov., Lactobacillus mellis sp. nov., Lactobacillus melliventris sp. nov., Lactobacillus kimbladii sp. nov., Lactobacillus helsingborgensis sp. nov. and Lactobacillus kullabergensis sp. nov. for these novel species, with the respective type strains being Fhon13N(T) ( = DSM 26257(T) = CCUG 63287(T)), Bin4N(T) ( = DSM 26254(T) = CCUG 63291(T)), Hon2N(T) ( = DSM 26255(T) = CCUG 63289(T)), Hma8N(T) ( = DSM 26256(T) = CCUG 63629(T)), Hma2N(T) ( = DSM 26263(T) = CCUG 63633(T)), Bma5N(T) ( = DSM 26265(T) = CCUG 63301(T)) and Biut2N(T) ( = DSM 26262(T) = CCUG 63631(T)).
我们之前在蜜蜂的蜜胃中发现了一种共生的乳酸菌(LAB)微生物群。该微生物群由几种双歧杆菌和乳杆菌的菌株组成。16S rRNA 基因序列分析以及表型和遗传特征表明,分离出的菌株代表了七个新种。其中一个与乳杆菌 kunkeei 聚为一类,而其他的则属于乳杆菌 buchneri 和乳杆菌 delbrueckii 亚群的乳杆菌。我们提议将这些新种分别命名为嗜酸乳杆菌 apinorum sp. nov.、蜜蜂乳杆菌 mellifer sp. nov.、乳杆菌 mellis sp. nov.、乳杆菌 melliventris sp. nov.、金氏乳杆菌 kimbladii sp. nov.、赫尔辛堡乳杆菌 helsingborgensis sp. nov. 和库尔贝格乳杆菌 kullabergensis sp. nov.,其相应的模式菌株为 Fhon13N(T)(=DSM 26257(T)=CCUG 63287(T))、Bin4N(T)(=DSM 26254(T)=CCUG 63291(T))、Hon2N(T)(=DSM 26255(T)=CCUG 63289(T))、Hma8N(T)(=DSM 26256(T)=CCUG 63629(T))、Hma2N(T)(=DSM 26263(T)=CCUG 63633(T))、Bma5N(T)(=DSM 26265(T)=CCUG 63301(T))和 Biut2N(T)(=DSM 26262(T)=CCUG 63631(T))。