Chen Ri-Dao, Duan Rui-Gang, Zou Jian-Hua, Li Jun-Wei, Liu Xiao-Yue, Wang Hai-Yan, Li Qiu-Hong, Dai Jun-Gui
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, China.
Institute of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jan;41(1):87-91. doi: 10.4268/cjcmm20160117.
Various chromatographic techniques, including silica gel column chromatography, Sephadex LH-20, preparative thin-layer chromatography, and preparative HPLC, were employed to isolate the chemical constituents from callus cultures of Dysosma versipellis. Structures of the compounds were elucidated based on UV, IR, MS and NMR spectroscopic data analysis. Totally, seven flavonoid glycosides were isolated from the 95% ethanol extract of the callus cultures and identified as kaempferol-3-O-[6″-(3″'-methoxy)-malonyl]-β-D-glucopyranoside(1), kaempferol-3-O-(6″-O-acetyl)-β-D-glucopyranoside(2), kaempferide-3-O-β-D-glucopyranoside(3), kaempferol-3-O-β-D-glucopyranoside(4), isoquercitrin(5), quercetin-4'-O-β-D-glucopyranoside(6) and kaempferol-3-(6″-malonyl)-β-D-glucopyranoside(7), respectively.All these compounds were isolated from callus cultures of D. versipellis for the first time.Compounds 1, 2, 3, 6 and 7 were firstly obtained from plant materials of D. versipellis, and compound 1 was a new compound.
采用多种色谱技术，包括硅胶柱色谱、葡聚糖凝胶LH-20、制备型薄层色谱和制备型高效液相色谱，从八角莲愈伤组织培养物中分离化学成分。基于紫外、红外、质谱和核磁共振光谱数据分析阐明了化合物的结构。从愈伤组织培养物的95%乙醇提取物中总共分离出7种黄酮苷，分别鉴定为山柰酚-3-O-[6″-(3″'-甲氧基)-丙二酰基]-β-D-吡喃葡萄糖苷(1)、山柰酚-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷(2)、山柰素-3-O-β-D-吡喃葡萄糖苷(3)、山柰酚-3-O-β-D-吡喃葡萄糖苷(4)、异槲皮苷(5)、槲皮素-4'-O-β-D-吡喃葡萄糖苷(6)和山柰酚-3-(6″-丙二酰基)-β-D-吡喃葡萄糖苷(7)。所有这些化合物均首次从八角莲愈伤组织培养物中分离得到。化合物1、2、3、6和7首次从八角莲植物材料中获得，化合物1为新化合物。
