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菌株HKF42胞外β-呋喃果糖苷酶的注释及从头测序表征

Annotation and De Novo Sequence Characterization of Extracellular β-Fructofuranosidase from Strain HKF42.

作者信息

Gujar Vaibhav V, Fuke Priya, Khardenavis Anshuman A, Purohit Hemant J

机构信息

1CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, India.

2Academy of Scientific and Innovative Research (AcSIR), CSIR-NEERI, Nagpur, 440020 India.

出版信息

Indian J Microbiol. 2018 Jun;58(2):227-233. doi: 10.1007/s12088-017-0704-y. Epub 2018 Jan 4.

DOI:10.1007/s12088-017-0704-y
PMID:29651183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5891470/
Abstract

The genome of a fungal strain strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.

摘要

能在20%蔗糖培养基上生长的真菌菌株HKF42的基因组已注释出7595个蛋白质编码序列。在挖掘碳水化合物活性酶（CAZymes）时，我们注释出一个负责合成低聚果糖（FOS，一种已知的益生元）的β-呋喃果糖苷酶基因。通过生成蔗果三糖和蔗果四糖形式的低聚果糖，证明并验证了该酶的活性。

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