菌株HKF2的基因组序列草图，HKF2是一种具有生产益生元合成酶潜力的真菌。
Draft genome sequence of strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes.
作者信息
Gujar Vaibhav V, Fuke Priya, Khardenavis Anshuman A, Purohit Hemant J
机构信息
1CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, India.
2Academy of Scientific and Innovative Research (AcSIR), CSIR-NEERI, Nehru Marg, Nagpur, 440020 India.
出版信息
3 Biotech. 2018 Feb;8(2):106. doi: 10.1007/s13205-018-1132-3. Epub 2018 Feb 1.
Abstract
In this study, we have characterized a novel set of extracellular enzymes produced by strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with followed by and . The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.
摘要
在本研究中，我们对菌株HKF2产生的一组新型胞外酶进行了表征。生成了一份31.5兆碱基对的基因组草图数据，注释显示共有11243个蛋白质编码基因，其中609个是碳水化合物活性酶（CAZymes），其中大部分被发现与[具体内容缺失]具有同源性，其次是[具体内容缺失]和[具体内容缺失]。在基因组草图中鉴定出的突出CAZyme基因编码参与益生元（如低聚果糖和低聚菊糖）生产的酶。相应的酶活性测定表明，该分离株分别具有产生11.8和3.8 U/mL的β-呋喃果糖苷酶和菊粉酶的潜力。本研究强调了污水处理厂作为分离具有益生元合成酶生产潜力真菌的新型且未充分探索的生态位的重要性。
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