1 Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg (FAU) , Erlangen, Germany .
2 Department of Plastic Surgery, Hand and Burn Surgery, University Hospital of Aachen, RWTH University of Aachen , Aachen, Germany .
Tissue Eng Part A. 2018 Sep;24(17-18):1320-1331. doi: 10.1089/ten.TEA.2017.0496. Epub 2018 May 9.
Vascularization of bioartificial tissues can be significantly enhanced by the generation of an arteriovenous (AV) loop. Besides the surgical vascularization, the choice of the scaffold and the applied cells are indispensable cofactors. The combination of alginate dialdehyde and gelatin (ADA-GEL) and mesenchymal stem cells (MSCs) is a promising approach with regard to biocompatibility, biodegradation, as well as de novo tissue formation. In this study, we targeted the investigation of the vascularization of ADA-GEL with and in the absence of encapsulated MSCs in the AV loop model. A Teflon chamber filled with ADA-GEL microcapsules was placed in the groin of Lewis rats and an AV loop was placed into the chamber. Group A encompassed the ADA-GEL without MSCs, whereas group B contained 2 × 10 DiI-labeled MSCs/mL ADA-GEL. Four weeks postoperatively, tissue formation and vascularization were investigated by histology and microcomputed tomography. We were able to prove vascularization originating from the AV loop in both groups with statistically significant more vessels in group B containing MSCs. Moreover, encapsulated MSCs promoted biodegradation of the ADA-GEL microcapsules. In the present study, we were able to demonstrate for the first time, the successful vascularization of ADA-GEL microcapsules by means of the AV loop. Furthermore, ADA-GEL displayed a good biocompatibility and encapsulation of MSCs into ADA-GEL microcapsule-enhanced vascularization as well as biodegradation.
生物人工组织的血管化可以通过生成动静脉（AV）环来显著增强。除了手术血管化外，支架的选择和应用的细胞也是不可或缺的协同因素。藻酸盐二醛和明胶（ADA-GEL）与间充质干细胞（MSCs）的组合在生物相容性、生物降解以及新组织形成方面具有广阔的应用前景。在这项研究中，我们的目的是研究在 AV 环模型中存在和不存在包封 MSC 的情况下 ADA-GEL 的血管化情况。将充满 ADA-GEL 微胶囊的特氟龙室置于 Lewis 大鼠的腹股沟处，并将 AV 环置于室内。A 组为不含 MSC 的 ADA-GEL，B 组为含有 2×10 DiI 标记 MSC/mL ADA-GEL。术后 4 周，通过组织学和微计算机断层扫描研究组织形成和血管化情况。我们能够证明在两组中都有源自 AV 环的血管化，其中含有 MSC 的 B 组的血管数量具有统计学意义。此外，包封的 MSC 促进了 ADA-GEL 微胶囊的生物降解。在本研究中，我们首次成功地通过 AV 环实现了 ADA-GEL 微胶囊的血管化。此外，ADA-GEL 表现出良好的生物相容性，并且将 MSC 包封到 ADA-GEL 微胶囊中可以增强血管化和生物降解。
