Emergency Department, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou, Changzhou, 213003, Jiangsu, China.
Department of Critical Care Medicine, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou, Changzhou, 213003, Jiangsu, China.
Inflammation. 2018 Aug;41(4):1290-1296. doi: 10.1007/s10753-018-0777-8.
Isoliquiritigenin (ILG), a major ingredient of licorice, has been reported to have anti-oxidative and anti-inflammatory effects. The aim of this study was to investigate the protective effects of ILG on lung injury using an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). Male BALB/c mice were conditioned with ILG 1 h before intranasal instillation of LPS. The effects of ILG on lung injury were assessed by measuring lung histopathological examination, MPO assay, wet/dry (W/D) ratio, and inflammatory cytokine production. The results showed that ILG significantly inhibited LPS-induced lung histopathological changes and the MPO activity. Meanwhile, it attenuated the wet/dry (W/D) ratio in the lung tissues. The results also indicated that ILG inhibited LPS-induced ALI in the expression of inflammatory cytokines in the BALF. Furthermore, ILG can decrease the activity of NF-κB and can increase the expression of PPAR-γ. These findings suggested that ILG inhibited the inflammatory of LPS-induced lung injury by activating PPAR-γ and inhibiting NF-κB activation.
甘草中的异甘草素（ILG）具有抗氧化和抗炎作用。本研究旨在通过脂多糖（LPS）诱导的急性肺损伤（ALI）动物模型，探讨 ILG 对肺损伤的保护作用。雄性 BALB/c 小鼠在鼻腔内滴注 LPS 前用 ILG 预处理 1 小时。通过测量肺组织病理学检查、髓过氧化物酶（MPO）测定、湿/干（W/D）比值和炎症细胞因子产生来评估 ILG 对肺损伤的影响。结果表明，ILG 显著抑制 LPS 诱导的肺组织病理学改变和 MPO 活性。同时，它减轻了肺组织中的湿/干（W/D）比值。结果还表明，ILG 抑制了 LPS 诱导的 ALI 中 BALF 中炎症细胞因子的表达。此外，ILG 可以降低 NF-κB 的活性并增加 PPAR-γ 的表达。这些发现表明，ILG 通过激活 PPAR-γ和抑制 NF-κB 激活来抑制 LPS 诱导的肺损伤的炎症反应。
