Department of Paediatrics, Affiliated Hospital of Jining Medical University, Jining, 272029, Shandong, China.
Inflammation. 2018 Dec;41(6):2012-2017. doi: 10.1007/s10753-018-0844-1.
Terpinen-4-ol, the major constituent of tea tree oil, has been reported to have anti-inflammatory effect. However, whether terpinen-4-ol could attenuate LPS-induced inflammation in lung tissues remains unclear. In the present study, we aimed to investigate the protective effects of terpinen-4-ol on LPS-induced acute lung injury (ALI) in mice. Terpinen-4-ol could inhibit LPS-induced ALI as confirmed by the decreased lung histopathological changes, MPO activity, and lung W/D ratio caused by terpinen-4-ol. The production of TNF-α and IL-1β in the BALF was suppressed by the treatment of terpinen-4-ol. Western blot analysis showed that terpinen-4-ol significantly attenuated LPS-induced phosphorylation of IκBα and NF-κB p65. Furthermore, the expression of PPAR-γ was dose-dependently upregulated by the treatment of terpinen-4-ol. In conclusion, the results of this study indicated that terpinen-4-ol inhibited LPS-induced ALI via activating PPAR-γ, which subsequently attenuated LPS-induced NF-κB activation and inflammatory response.
萜品-4-醇是茶树油的主要成分，据报道具有抗炎作用。然而，萜品-4-醇是否能减轻 LPS 诱导的肺组织炎症尚不清楚。在本研究中，我们旨在探讨萜品-4-醇对 LPS 诱导的急性肺损伤（ALI）的保护作用。萜品-4-醇可抑制 LPS 诱导的 ALI，这可通过萜品-4-醇降低肺组织病理学变化、髓过氧化物酶（MPO）活性和肺湿/干重比来证实。萜品-4-醇处理可抑制 BALF 中 TNF-α 和 IL-1β 的产生。Western blot 分析表明，萜品-4-醇可显著减轻 LPS 诱导的 IκBα 和 NF-κB p65 的磷酸化。此外，萜品-4-醇处理可剂量依赖性地上调 PPAR-γ 的表达。总之，本研究结果表明，萜品-4-醇通过激活 PPAR-γ 抑制 LPS 诱导的 ALI，从而减轻 LPS 诱导的 NF-κB 激活和炎症反应。
