Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Department of Pharmacology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
Inflammation. 2018 Aug;41(4):1259-1265. doi: 10.1007/s10753-018-0773-z.
Acute lung injury (ALI) is a serious disease with morbidity and mortality in patients. Engeletin (dihydrokaempferol 3-rhamnoside) is a flavanonol glycoside. It can be found in the skin of white grapes and white wine and is widely distributed in southeast Asia. In our study, we evaluated the protective and therapeutic effects of engeletin on lipopolysaccharide (LPS)-induced ALI in animal model. We determined the level of peroxisome proliferator-activated receptor-γ (PPAR-γ), nuclear factor kappaB (NF-κB), and IκBα by western blotting. The myeloperoxidase (MPO) activity and lung wet/dry (W/D) ratio in lung tissues were also detected. Histopathological changes and the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β were determined by H&E staining and ELISA. The MPO activity and lung W/D ratio induced by LPS were attenuated by engeletin. The numbers of inflammatory cells and the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were ameliorated by engeletin. Furthermore, the results also showed that engeletin significantly suppressed LPS-induced NF-κB activation. The expression of PPAR-γ was upregulated by treatment of engeletin. In conclusion, we found that engeletin had protective and therapeutic effects against LPS-induced ALI by activating PPAR-γ. Engeletin is a potentially effective agent for the treatment of lung injury.
急性肺损伤（ALI）是一种严重的疾病，患者的发病率和死亡率都很高。山柰素（二氢山柰酚 3-鼠李糖苷）是一种黄烷醇糖苷。它可以在白葡萄和白葡萄酒的果皮中找到，广泛分布于东南亚。在我们的研究中，我们评估了山柰素对脂多糖（LPS）诱导的动物模型中 ALI 的保护和治疗作用。我们通过 Western blot 测定过氧化物酶体增殖物激活受体-γ（PPAR-γ）、核因子 kappaB（NF-κB）和 IκBα的水平。还检测了肺组织中髓过氧化物酶（MPO）活性和肺湿/干（W/D）比值。通过 H&E 染色和 ELISA 测定组织病理学变化和促炎细胞因子 TNF-α、IL-6 和 IL-1β。山柰素减弱了 LPS 诱导的 MPO 活性和肺 W/D 比值。山柰素改善了支气管肺泡灌洗液（BALF）中炎症细胞的数量和炎症细胞因子的水平。此外，结果还表明，山柰素通过抑制 NF-κB 的激活显著抑制了 LPS 诱导的 ALI。山柰素处理后 PPAR-γ 的表达上调。总之，我们发现山柰素通过激活 PPAR-γ对 LPS 诱导的 ALI 具有保护和治疗作用。山柰素是一种治疗肺损伤的潜在有效药物。
