Schripsema Jan, Dagnino Denise
Grupo Metabolômica, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
Methods Mol Biol. 2018;1738:195-202. doi: 10.1007/978-1-4939-7643-0_13.
Metabolomics is the area of research, which strives to obtain complete metabolic fingerprints, to detect differences between them, and to provide hypothesis to explain those differences [1]. But obtaining complete metabolic fingerprints is not an easy task. Metabolite extraction is a key step during this process, and much research has been devoted to finding the best solvent mixture to extract as much metabolites as possible.Here a procedure is described for analysis of both polar and apolar metabolites using a two-phase extraction system. DO and CDCl are the solvents of choice, and their major advantage is that, for the identification of the compounds, standard databases can be used because DO and CDCl are the solvents most commonly used for pure compound NMR spectra. The procedure enables the absolute quantification of components via the addition of suitable internal standards. The extracts are also suitable for further analysis with other systems like LC-MS or GC-MS.
代谢组学是一个研究领域，致力于获取完整的代谢指纹图谱，检测它们之间的差异，并提供假设来解释这些差异[1]。但获取完整的代谢指纹图谱并非易事。代谢物提取是这一过程中的关键步骤，许多研究致力于寻找最佳溶剂混合物以尽可能多地提取代谢物。本文描述了一种使用两相提取系统分析极性和非极性代谢物的方法。氘代水（D₂O）和氘代氯仿（CDCl₃）是首选溶剂，它们的主要优点是，对于化合物的鉴定，可以使用标准数据库，因为D₂O和CDCl₃是纯化合物核磁共振谱最常用的溶剂。该方法通过添加合适的内标实现成分的绝对定量。提取物也适用于用其他系统如液相色谱 - 质谱联用（LC - MS）或气相色谱 - 质谱联用（GC - MS）进行进一步分析。
