Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, Texas, 79968.
Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas, 78712.
J Biomed Mater Res B Appl Biomater. 2019 Feb;107(2):314-323. doi: 10.1002/jbm.b.34123. Epub 2018 Apr 15.
Three-dimensional bioprinting is an innovative technique in tissue engineering, to create layer-by-layer structures, required for mimicking body tissues. However, synthetic bioinks do not generally possess high printability and biocompatibility at the same time. So, there is an urgent need for naturally derived bioinks that can exhibit such optimized properties. We used furfuryl-gelatin as a novel, visible-light crosslinkable bioink for fabricating cell-laden structures with high viability. Hyaluronic acid was added as a viscosity enhancer and either Rose Bengal or Riboflavin was used as a visible-light crosslinker. Crosslinking was done by exposing the printed structure for 2.5 min to visible light and confirmed using Fourier transform infrared spectroscopy and rheometry. Scanning electron microscopy revealed a highly porous networked structure. Three different cell types were successfully bioprinted within these constructs. Mouse mesenchymal stem cells printed within monolayer and bilayer sheets showed viability, network formation and proliferation (∼5.33 times) within 72 h of culture. C2C12 and STO cells were used to print a double layered structure, which showed evidence of the viability of both cells and heterocellular clusters within the construct. This furfuryl-gelatin based bioink can be used for tissue engineering of complex tissues and help in understanding how cellular crosstalk happens in vivo during normal or diseased pathology. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 314-323, 2019.
三维生物打印是组织工程中的一项创新技术，可逐层构建结构，以模拟人体组织。然而，合成生物墨水通常不能同时具有高可打印性和生物相容性。因此，迫切需要能够表现出这种优化特性的天然衍生生物墨水。我们使用糠基-明胶作为一种新型的可见光交联生物墨水，用于制造具有高活力的细胞负载结构。透明质酸被添加作为粘度增强剂，并且孟加拉玫瑰红或核黄素被用作可见光交联剂。通过将打印结构暴露于可见光下 2.5 分钟来进行交联，并使用傅里叶变换红外光谱和流变学进行确认。扫描电子显微镜显示出高度多孔的网状结构。三种不同的细胞类型成功地在这些构建体中进行了生物打印。在培养 72 小时内，打印在单层和双层薄片中的小鼠间充质干细胞表现出活力、网络形成和增殖（约 5.33 倍）。C2C12 和 STO 细胞用于打印双层结构，该结构显示出构建体中两种细胞和异细胞簇的活力证据。这种基于糠基-明胶的生物墨水可用于复杂组织的组织工程，并有助于理解在正常或患病病理过程中细胞串扰如何在体内发生。© 2018 威利父子公司。J 生物医学材料研究部分 B：应用生物材料，107B:314-323,2019.
