Anil Kumar Shweta, Tasnim Nishat, Dominguez Erick, Allen Shane, Suggs Laura J, Ito Yoshihiro, Joddar Binata
Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, 500 W University Avenue, El Paso, TX 79968, USA.
Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712, USA.
Gels. 2018 Sep 4;4(3):73. doi: 10.3390/gels4030073.
3D bioprinting holds great promise in the field of regenerative medicine as it can create complex structures in a layer-by-layer manner using cell-laden bioinks, making it possible to imitate native tissues. Current bioinks lack both high printability and biocompatibility required in this respect. Hence, the development of bioinks that exhibit both properties is needed. In our previous study, a furfuryl-gelatin-based bioink, crosslinkable by visible light, was used for creating mouse mesenchymal stem cell-laden structures with a high fidelity. In this study, lattice mesh geometries were printed in a comparative study to test against the properties of a traditional rectangular-sheet. After 3D printing and crosslinking, both structures were analysed for swelling and rheological properties, and their porosity was estimated using scanning electron microscopy. The results showed that the lattice structure was relatively more porous with enhanced rheological properties and exhibited a lower degradation rate compared to the rectangular-sheet. Further, the lattice allowed cells to proliferate to a greater extent compared to the rectangular-sheet, which initially retained a lower number of cells. All of these results collectively affirmed that the lattice poses as a superior scaffold design for tissue engineering applications.
3D生物打印在再生医学领域具有巨大潜力，因为它可以使用含细胞的生物墨水逐层创建复杂结构，从而有可能模仿天然组织。目前的生物墨水在这方面缺乏所需的高打印性和生物相容性。因此，需要开发兼具这两种特性的生物墨水。在我们之前的研究中，一种基于糠醇-明胶的生物墨水可通过可见光交联，用于创建具有高保真度的含小鼠间充质干细胞结构。在本研究中，通过对比研究打印晶格网状几何结构，以测试其与传统矩形片材性能的差异。3D打印和交联后，对两种结构进行了溶胀和流变性能分析，并使用扫描电子显微镜估计其孔隙率。结果表明，与矩形片材相比，晶格结构孔隙率相对更高，流变性能增强，降解速率更低。此外，与最初保留较少细胞的矩形片材相比，晶格结构能使细胞增殖到更大程度。所有这些结果共同证实，晶格结构是组织工程应用中一种更优的支架设计。
