Refractories, Ceramics and Building materials Department, National Research Centre(NRC), 33 El Behooth St., Dokki, Giza, 12622, Egypt.
Department of Narcotics and Poisons Pharmacology and Toxicology, National Research Centre(NRC), 33 El Behooth St., Dokki, Giza, 12622, Egypt.
J Biomed Mater Res B Appl Biomater. 2019 Feb;107(2):388-399. doi: 10.1002/jbm.b.34130. Epub 2018 Apr 15.
Copper (Cu)-doped calcium silicate nanoparticles were synthesized by a wet precipitation method as economical bone fracture filler. The aim was to improve the overall physicochemical properties, bioactivity, and biological performance of the bone fracture filler prepared herein. The synthesized nanoparticles were evaluated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). The bioactivity of the prepared nanoparticles was investigated after immersion in simulated body fluid (SBF) by means of inductively coupled plasma (ICP), SEM coupled with energy dispersive X-rays (EDX), and FTIR. The size and bioactivity of the prepared nanoparticles after 15 days of immersion in SBF was dependent on the Cu concentrations. The fracture healing ability of the fabricated nanoparticles on adult aged male Wistar rats was enhanced by the presence of copper. All the obtained results are of high relevance for fabricating improved Cu-doped calcium silicate nanoparticles (∼50 nm) as low cost bone fracture filler. In addition, the in vivo study presented complete healing of the tibiae bone with normal architecture of bone tissue specifically calcium silicate nanoparticles doped with 3% and 5% Cu. Hence, the presence of copper is a promising tactic for improving the biological properties of calcium silicate. Therefore, the designed nanoparticles have huge potential for the treatment of bone fractures. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 388-399, 2019.
铜（Cu）掺杂的硅酸钙纳米粒子通过湿沉淀法合成，用作经济的骨折填充剂。目的是改善本文制备的骨折填充剂的整体物理化学性质、生物活性和生物学性能。使用 X 射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）对合成的纳米粒子进行了评估。通过电感耦合等离子体（ICP）、SEM 与能谱（EDX）和 FTIR 研究了在模拟体液（SBF）中浸泡后的纳米粒子的生物活性。在 SBF 中浸泡 15 天后，纳米粒子的尺寸和生物活性取决于 Cu 的浓度。铜的存在增强了制备的纳米粒子在成年雄性 Wistar 大鼠上的骨折愈合能力。所有获得的结果对于制备具有改进的 Cu 掺杂硅酸钙纳米粒子（约 50nm）作为低成本骨折填充剂都具有重要意义。此外，体内研究表明，掺杂 3%和 5%Cu 的硅酸钙纳米粒子可使胫骨完全愈合，并且具有正常的骨组织结构。因此，铜的存在是改善硅酸钙生物性能的一种有前途的策略。因此，设计的纳米粒子在治疗骨折方面具有巨大的潜力。©2018 威利父子公司。生物医学材料研究杂志 B：应用生物材料，107B:388-399，2019。
