Taha Said K, Abdel Hamid Mohamed A, Hamzawy Esmat M A, Kenawy Sayed H, El-Bassyouni Gehan T, Hassan Elham A, Tarek Heba E
Surgery and Oral Medicine Department, Oral and Dental Research Institute, National Research Centre, 33 El Buhouth St, Dokki, Giza 12622, Egypt.
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Saudi Dent J. 2022 Sep;34(6):485-493. doi: 10.1016/j.sdentj.2022.06.008. Epub 2022 Jun 28.
To evaluate bioactivity and osteogenic potential of calcium silicate (CS)-doped iron oxide (FeO) nanoparticles versus pure CS in the reconstruction of induced critical-sized mandibular defects.
CS-doped FeO was prepared; morphological and microstructure identification of nanoparticles were made. An randomised design was developed on 24 adult male dogs where four critical-sized mandibular defects were created in each dog. Bone defects were allocated into control, CS, CS-3% FeO and CS-10% FeO group. Dogs were euthanized at 1 and 3 months (12 dog/time) for histopathologic and histomorphometric evaluation.
At three months, bone formation and maturation were evident where mean ± SD percent of mature bone was 2.66 ± 1.8, 9.9 ± 2.5, 22.9 ± 4.9, and 38.6 ± 8.1 in control, CS, CS-3% FeO, and CS-10% FeO groups respectively. A high significant ( < 0.001) increase in area percent of mature bone was recorded in CS, CS-3% FeO, and CS- 10% FeO groups compared to control group (73%, 88% and 93.3% respectively). Significant increase ( < 0.001) in area of mature bone was recorded in CS-3% FeO and CS-10% FeO groups compared to CS group. A significant increase ( < 0.001) in area of mature bone formation was detected in CS-10% FeO group compared to other groups.
CS-doped FeO has good osteoconductive, biocompatible properties with promoted bone regeneration. FeO has synergistic effect in combination with CS to promote bone formation. Increasing concentration of FeO nanoparticles resulted in improved osteogenesis and maturation. Results suggests that the novel CS-FeO alloplasts could be used for reconstruction of critical-sized bone defects.
评估掺杂硅酸钙（CS）的氧化铁（FeO）纳米颗粒与纯CS相比，在诱导临界尺寸下颌骨缺损重建中的生物活性和成骨潜力。
制备掺杂CS的FeO；对纳米颗粒进行形态和微观结构鉴定。对24只成年雄性犬进行随机设计，每只犬制造4个临界尺寸的下颌骨缺损。将骨缺损分为对照组、CS组、CS - 3% FeO组和CS - 10% FeO组。在1个月和3个月时对犬实施安乐死（每次12只犬），进行组织病理学和组织形态计量学评估。
在3个月时，骨形成和成熟明显，对照组、CS组、CS - 3% FeO组和CS - 10% FeO组成熟骨的平均±标准差百分比分别为2.66±1.8、9.9±2.5、22.9±4.9和38.6±8.1。与对照组相比，CS组、CS - 3% FeO组和CS - 10% FeO组成熟骨面积百分比显著增加（<0.001）（分别为73%、88%和93.3%）。与CS组相比，CS - 3% FeO组和CS - 10% FeO组成熟骨面积显著增加（<0.001）。与其他组相比，CS - 10% FeO组成熟骨形成面积显著增加（<0.001）。
掺杂CS的FeO具有良好的骨传导性、生物相容性，能促进骨再生。FeO与CS联合具有协同作用，可促进骨形成。增加FeO纳米颗粒浓度可改善成骨和成熟。结果表明新型CS - FeO异体骨可用于临界尺寸骨缺损的重建。
