Won Sangho, Hindmarsh Steven, Gibson Matthew I
Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, U.K.
Department of Physics, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, U.K.
ACS Macro Lett. 2018 Feb 20;7(2):178-183. doi: 10.1021/acsmacrolett.7b00891. Epub 2018 Jan 18.
Carbohydrate-carbohydrate interactions are proposed to be biologically significant but have lower affinities than the well-studied carbohydrate-protein interactions. Here we introduce multivalent glyconanostructures where the surface expression of lactose can be triggered by an external stimulus, and a gold nanoparticle core enables colorimetric signal outputs to probe binding. Macromolecular engineering of a responsive polymer "gate" enables the lactose moieties to be presented only when an external stimulus is present, mimicking how nature uses enzymes to dynamically regulate glycan expression. Two different carbohydrate-carbohydrate interactions are investigated using this tool.
碳水化合物与碳水化合物之间的相互作用被认为具有生物学意义，但与经过充分研究的碳水化合物与蛋白质之间的相互作用相比，其亲和力较低。在此，我们引入了多价糖纳米结构，其中乳糖的表面表达可由外部刺激触发，并且金纳米颗粒核心能够实现比色信号输出以探测结合情况。一种响应性聚合物“门”的大分子工程使得乳糖部分仅在存在外部刺激时才会呈现，这模仿了自然界利用酶来动态调节聚糖表达的方式。利用该工具研究了两种不同的碳水化合物与碳水化合物之间的相互作用。
