Aliouche Nadia, Sifour Mohamed, Kebsa Widad, Khennouf Tarek, Ercan Feriha, Ouled-Haddar Houria
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.
Laboratory of Biotechnology, Environment and Health, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.
Probiotics Antimicrob Proteins. 2024 Jul 15. doi: 10.1007/s12602-024-10321-4.
Lactiplantibacillus plantarum (Lpb. plantarum), as a safe probiotic microorganism, has been documented for its production of multiple bioactive compounds, such as exopolysaccharides (EPS), which have been used in the treatment of many gastrointestinal diseases, including gastric ulcers. The present study aims to investigate the prophylactic and antiulcerogenic effects of the potential probiotic Lbp. plantarum E1K2R2 and its EPS against ibuprofen-induced gastric ulcer. A gastric ulcer model was established by feeding fasted rats with ibuprofen at a single dose (200 mg/kg body weight). The Lpb. plantarum E1K2R2 (10 CFU), its EPS (200 mg/kg bw), and the anti-ulcer reference drug (omeprazole) (20 mg/kg bw) were separately used to feed rats for seven consecutive days before ibuprofen administration. The mechanisms were meticulously examined, focusing on the anti-secretory activity and mucus production as well as the anti-inflammatory and antioxidant activities. The findings revealed that the gastro-preventive effect of Lbp. plantarum E1K2R2 (88.43%) was higher than that of the EPS (66.26%) and close to that of omeprazole (89.87%). This effect was achieved through similar mechanisms, including regulation of the secretory activity, augmentation of mucus production, mitigation of inflammation, and enhancement of the gastric mucosa's antioxidant capacity. Moreover, it was found that Lbp. plantarum E1K2R2 and its EPS induce the activities of gastric antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and S-transferase (GST); enhance glutathione (GSH) content; and reduce mucosal nitric oxide (NO), myeloperoxidase (MPO), and malondialdehyde (MDA) levels. Furthermore, histopathological and hematological examinations confirmed that both pre-treatments could effectively maintain the structural integrity of the gastric mucosa and improve some hematological parameters, respectively. This implies that Lpb. plantarum E1K2R2 and its EPS possess the potential to counteract ibuprofen-associated gastric ulcers, leveraging a variety of protective mechanisms.
植物乳杆菌（Lpb. plantarum）作为一种安全的益生菌微生物，已被证明能产生多种生物活性化合物，如胞外多糖（EPS），这些化合物已被用于治疗多种胃肠道疾病，包括胃溃疡。本研究旨在探讨潜在益生菌植物乳杆菌E1K2R2及其EPS对布洛芬诱导的胃溃疡的预防和抗溃疡作用。通过给禁食大鼠单次喂食布洛芬（200 mg/kg体重）建立胃溃疡模型。在给予布洛芬之前，分别用植物乳杆菌E1K2R2（10 CFU）、其EPS（200 mg/kg体重）和抗溃疡参考药物（奥美拉唑）（20 mg/kg体重）连续7天喂养大鼠。对其作用机制进行了细致研究，重点关注抗分泌活性、黏液产生以及抗炎和抗氧化活性。研究结果显示，植物乳杆菌E1K2R2的胃预防效果（88.43%）高于EPS（66.26%），且接近奥美拉唑（89.87%）。这种效果是通过类似的机制实现的，包括调节分泌活性、增加黏液产生、减轻炎症以及增强胃黏膜的抗氧化能力。此外，还发现植物乳杆菌E1K2R2及其EPS可诱导胃抗氧化酶的活性：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和S-转移酶（GST）；提高谷胱甘肽（GSH）含量；并降低黏膜一氧化氮（NO）、髓过氧化物酶（MPO）和丙二醛（MDA）水平。此外，组织病理学和血液学检查证实，两种预处理均可有效维持胃黏膜的结构完整性，并分别改善一些血液学参数。这表明植物乳杆菌E1K2R2及其EPS具有利用多种保护机制对抗布洛芬相关胃溃疡的潜力。
