Enzyme and Microbial Technology Research Centre, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia; Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Enzyme and Microbial Technology Research Centre, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia; Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Bioelectrochemistry. 2018 Aug;122:199-205. doi: 10.1016/j.bioelechem.2018.04.004. Epub 2018 Apr 9.
Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91ngmL to 125.00ngmL with lowest limit of detection (LOD) of 3.80ngmL at 3σm. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.
乙型肝炎病毒核心抗原（HBcAg）是乙型肝炎病毒（HBV）的主要结构蛋白。血清中抗-HBcAg 抗体的存在表明一个人已经接触过 HBV。本研究表明，将 HBcAg 固定在金纳米粒子修饰的还原氧化石墨烯（rGO-en-AuNPs）纳米复合材料上，可以用作一种抗原功能化表面，以检测抗-HBcAg 的存在。然后，允许修饰后的 rGO-en-AuNPs/HBcAg 与抗雌二醇抗体和牛血清白蛋白一起进行抗-HBcAg 的阻抗检测作为干扰。在成功检测到加标缓冲液样品中的抗-HBcAg 后，进一步在加标人血清样品中进行了抗体的阻抗检测。电化学响应表明，电子转移电阻与抗-HBcAg 浓度之间呈线性关系，范围从 3.91ngmL 到 125.00ngmL，检测下限（LOD）为 3.80ngmL，在 3σm 时。该方法具有快速、简便的特点，有望成为检测抗-HBcAg 的一种方法。
