Castro David J, Cerezo Isabel, Sampedro Inmaculada, Martínez-Checa Fernando
Microbial Exopolysaccharide Research Group, Department of Microbiology, Pharmacy Faculty, Campus de Cartuja s/n, 18071 Granada, Spain.
Biomedical Research Center (CIBM), Biotechnology Institute, Avda del Conocimiento s/n, 18100, Armilla, Granada, Spain.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1851-1856. doi: 10.1099/ijsem.0.002744. Epub 2018 Apr 17.
Strain D15 was isolated from a soil sample taken from Rambla Salada (Murcia), south-eastern Spain, by using the dilution-to-extinction method. The strain, a Gram-stain-negative aerobic bacteria, is non-motile, ovoid- or rod-shaped, catalase- and oxidase-positive, and grows at NaCl concentrations within the range 0.5-10 % (w/v) [optimum 3 % (w/v)], at 5-30 °C (optimum 28 °C) and at pH 6-9 (optimum pH 7.0). The 16S rRNA gene sequence indicates that it belongs to the genus Roseovarius in the class Alphaproteobacteria. Its closest relatives are Roseovarius tolerans EL-172 and Roseovarius azorensis SSW084, to which the strain shows 16S rRNA gene-sequence similarity values of 96.1 and 95.3 %, respectively. The DNA G+C content is 63 mol%. The major fatty acids (>5 % of the total fatty acids) of strain D15 are C18 : 1ω7c, C16 : 0 and C12 : 0. The only detected isoprenoid quinone of strain D15 is ubiquinone 10 (Q-10). The polar lipid profile contains phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus Roseovarius, for which the name Roseovarius ramblicola sp. nov. is proposed. Strain D15 (=CECT 9424=LMG 30322) is the type strain.
菌株D15是通过稀释至灭绝法从西班牙东南部兰布拉萨拉达（穆尔西亚）采集的土壤样本中分离得到的。该菌株为革兰氏阴性需氧菌，无运动性，呈卵形或杆状，过氧化氢酶和氧化酶呈阳性，在NaCl浓度为0.5-10%（w/v）[最适浓度为3%（w/v）]、温度为5-30°C（最适温度为28°C）、pH值为6-9（最适pH值为7.0）的条件下生长。16S rRNA基因序列表明它属于α-变形菌纲中的玫瑰色菌属。其最相近的亲缘种是耐盐玫瑰色菌EL-172和亚速尔玫瑰色菌SSW084，该菌株与它们的16S rRNA基因序列相似性值分别为96.1%和95.3%。DNA G+C含量为63 mol%。菌株D15的主要脂肪酸（占总脂肪酸的>5%）为C18:1ω7c、C16:0和C12:0。菌株D15唯一检测到的类异戊二烯醌是泛醌10（Q-10）。极性脂质谱包含磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、氨基脂质和三种极性脂质。基于系统发育、基因型、表型和化学分类学数据，该菌株代表玫瑰色菌属的一个新物种，为此提出玫瑰色盐沼玫瑰色菌新种（Roseovarius ramblicola sp. nov.）。菌株D15（=CECT 9424=LMG 30322）为模式菌株。
