Fujian Province University Technology and Engineering Center for Marine Biomedical Resource Utilization, Xiamen Medical College, Xiamen, 361023, PR China.
Xiamen Key Laboratory of Marine Medicinal Natural Products and Cell Engineering, Xiamen Medical College, Xiamen, PR China.
Int J Syst Evol Microbiol. 2020 Jan;70(1):274-281. doi: 10.1099/ijsem.0.003750.
A taxonomic study was carried out on strain HN-E21, which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E21 were Gram-stain-negative, non-spore-forming, tumbling-motile, ovoid- to rod-shaped and pale-yellow-pigmented that could grow at 15-42 °C (optimum, 37 °C), at pH 6-11 (pH 7) and in 0-14 % (w/v) NaCl (2-3 %). This isolate was positive for oxidase and catalase, but negative for hydrolysis of aesculin and gelatin. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HN-E21 formed a clade with BH-SD19, GRP21, M-S13-148, CAU1059 and NH52J within the family . Strain HN-E21 shared the highest similarity to BH-SD19 (95.5 %), followed by 112 (95.3 %), D1-W8 (95.2 %), YM-20 (95.0 %) and H50 (95.0 %). The dominant fatty acids were summed feature 8 (Cω7 and/or Cω6) and C The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, three unidentified lipids and one unidentified phospholipid. The respiratory quinone was identified as Q-10. The G+C content of the genomic DNA was 66.4 mol%. Based on the phenotypic and phylogenetic data, strain HN-E21 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E21 (=MCCC 1K03333=LMG 30456).
对一株从中国海南杨浦湾采集的海绵中分离得到的菌株 HN-E21 进行了分类学研究。菌株 HN-E21 的细胞革兰氏阴性，不形成孢子，翻滚运动，呈卵形到杆状，颜色为浅黄色，能在 15-42°C（最佳温度为 37°C）、pH 6-11（pH 7）和 0-14%（w/v）NaCl（2-3%）中生长。该分离株氧化酶和过氧化氢酶阳性，但不能水解马栗树皮苷和明胶。基于 16S rRNA 基因序列的系统发育树显示，菌株 HN-E21 与 BH-SD19、GRP21、M-S13-148、CAU1059 和 NH52J 一起形成了一个分支，属于. 菌株 HN-E21 与 BH-SD19 的相似度最高（95.5%），其次是 112（95.3%）、D1-W8（95.2%）、YM-20（95.0%）和 H50（95.0%）。主要脂肪酸为 summed feature 8（Cω7 和/或 Cω6）和 C 16:0 1-OH。主要极性脂包括磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、三种未鉴定的脂类和一种未鉴定的磷脂。呼吸醌为 Q-10。基因组 DNA 的 G+C 含量为 66.4mol%。基于表型和系统发育数据，菌株 HN-E21 代表了. 属的一个新种，建议采用新种名. sp. nov.，其模式菌株为 HN-E21（=MCCC 1K03333=LMG 30456）。
