Lu Lu-Yao, Chen Ling-Ling, Xing Zi-Yu, Hu Ting, Lu Jia-Sen, Huang Xing
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2018 Jun;68(6):1902-1906. doi: 10.1099/ijsem.0.002763. Epub 2018 Apr 17.
A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China. Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3 was most closely related to Paenibacillusripae HZ1 (96.8 %), followed by Paenibacillussputi KIT00200-70066-1 (94.7 %). The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0. The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%. The major polar lipids of strain J-3 were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. On the basis of genotypic and phenotypic data, strain J-3 represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp. nov. is proposed. The type strain is J-3 (=KCTC 33926=CGMCC 1.16455).
从中国江苏省盐城市采集的农田土壤中分离出一株革兰氏可变、短杆状、具运动性、产芽孢、严格需氧且耐碱的细菌，命名为菌株J-3。该菌株在30 °C、pH 7.0 - 8.0和0.5 %（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株J-3与河岸类芽孢杆菌HZ1（相似度96.8 %）关系最为密切，其次是痰液类芽孢杆菌KIT00200 - 70066 - 1（相似度94.7 %）。主要细胞脂肪酸为anteiso-C15 : 0和C16 : 0。主要呼吸醌为甲基萘醌-7，DNA G+C含量为41.2 mol%。菌株J-3的主要极性脂为氨基脂、磷脂、双磷脂酰甘油、磷脂酰甘油、磷酸氨基脂和磷脂酰乙醇胺。细胞壁肽聚糖的诊断性二氨基酸为内消旋二氨基庚二酸。基于基因型和表型数据，菌株J-3代表类芽孢杆菌属的一个新物种，为此提出新名称盐城类芽孢杆菌（Paenibacillus yanchengensis sp. nov.）。模式菌株为J-3（=KCTC 33926=CGMCC 1.16455）。
