Chen Zhaojin, Ouyang Wenkai, Chen Yan, Tian Wei, Sun Leni
Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North Diversion Project of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China.
School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1149-1154. doi: 10.1099/ijsem.0.003288. Epub 2019 Feb 15.
A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3, was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China. Strain 3-5-3 was oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3 was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2 (96.5 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3 and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %. The genomic G+C content was 53.8 mol%. Anteiso-C15 : 0 was the major fatty acid and MK-7 was the only menaquinone. The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids. On the basis of the results obtained in this study, strain 3-5-3 is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp. nov. is proposed. The type strain is 3-5-3 (=CGMCC 1.13686=KCTC 33998).
从中国河南省南阳市镉污染的玉米种植土壤中分离出一株革兰氏阴性、杆状、具运动性的细菌菌株，命名为3-5-3。菌株3-5-3氧化酶和过氧化氢酶呈阳性。基于16S rRNA基因序列的系统发育分析表明，菌株3-5-3属于类芽孢杆菌属，与厌氧类芽孢杆菌MH2关系最为密切（相似度为96.5%）。3-5-3与相近物种之间的平均核苷酸一致性和数字DNA-DNA杂交值分别为69.4-84.5%和18.1-18.4%。基因组G+C含量为53.8 mol%。anteiso-C15:0是主要脂肪酸，MK-7是唯一的甲基萘醌。细胞壁肽聚糖中的二氨基酸含有内消旋二氨基庚二酸。极性脂质谱包含双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甲基乙醇胺、两种未鉴定的糖脂、两种未鉴定的氨基脂、一种未鉴定的磷脂、一种未鉴定的磷酸氨基脂和六种未鉴定的脂质。基于本研究获得的结果，菌株3-5-3被认为代表类芽孢杆菌属的一个新物种，为此提出名称类芽孢杆菌泽伊土壤杆菌新种。模式菌株为3-5-3（=CGMCC 1.13686=KCTC 33998）。
