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肌动蛋白结合蛋白 2 可作为预测鼻咽癌放疗反应的标志物，也是鼻咽癌潜在的治疗靶点。

Cofilin-2 Acts as a Marker for Predicting Radiotherapy Response and Is a Potential Therapeutic Target in Nasopharyngeal Carcinoma.

机构信息

Department of Radiation Oncology, Affiliated Cancer Hospital of Guangxi Medical University and Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China (mainland).

Guangxi Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumors, Guangxi Medical University, Nanning, Guangxi, China (mainland).

出版信息

Med Sci Monit. 2018 Apr 17;24:2317-2329. doi: 10.12659/msm.909832.

DOI:10.12659/msm.909832
PMID:29664897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5921956/
Abstract

BACKGROUND The purpose of this study was to determine whether cofilin-2 could serve as a protein marker for predicting radiotherapy response and as a potential therapeutic target in nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS Cofilin-2 protein levels in serum and tissue samples from patients with NPC were assessed by sandwich ELISA and IHC. In vitro, cofilin-2 levels in CNE-2R cells were significantly higher than those of CNE-2 cells. Meanwhile, CNE-2R cells were silenced for cofilin-2 to obtain a stable cofilin-2-RNAi-LV3 cell line. Then, cell proliferation, radiosensitivity, invasion and migration abilities, cell cycle, and apoptosis were evaluated by Cell Counting Kit 8 assay (CCK-8), flow cytometry (FCM), clone formation assay, and in vitro. RESULTS The secreted levels of the cofilin-2 protein in radioresistant NPC patients were significantly higher than those of radiosensitive cases. After cofilin-2 knockdown in nasopharyngeal carcinoma CNE-2R cells, proliferation was decreased, while apoptosis and radiosensitivity were enhanced; cell cycle distribution was altered, and the transplanted tumors in nude mice grew significantly less. CONCLUSIONS Overall, our findings suggest that cofilin-2 acts as a marker for predicting radiotherapy response and is a potential therapeutic target in nasopharyngeal carcinoma.

摘要

背景

本研究旨在确定肌动蛋白结合蛋白 2（Cofilin-2）能否作为预测鼻咽癌放疗反应的蛋白标志物和潜在的治疗靶点。

材料与方法

采用夹心酶联免疫吸附试验（ELISA）和免疫组化（IHC）检测鼻咽癌患者血清和组织样本中的 Cofilin-2 蛋白水平。体外实验中，CNE-2R 细胞中的 Cofilin-2 水平明显高于 CNE-2 细胞。同时，沉默 CNE-2R 细胞中的 Cofilin-2，获得稳定的 Cofilin-2-RNAi-LV3 细胞系。然后，通过细胞计数试剂盒 8 检测（CCK-8）、流式细胞术（FCM）、克隆形成实验和体外实验评估细胞增殖、放射敏感性、侵袭和迁移能力、细胞周期和细胞凋亡。

结果

放射抵抗的鼻咽癌患者的分泌型 Cofilin-2 蛋白水平明显高于放射敏感的患者。在鼻咽癌 CNE-2R 细胞中敲低 Cofilin-2 后，增殖减少，而凋亡和放射敏感性增强；细胞周期分布改变，裸鼠移植瘤的生长明显减少。

结论

总之，我们的研究结果表明，Cofilin-2 可作为预测放疗反应的标志物，也是鼻咽癌的潜在治疗靶点。

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