Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.
Tissue Eng Regen Med. 2020 Feb;17(1):91-103. doi: 10.1007/s13770-019-00232-9. Epub 2020 Jan 22.
The effect of nano controlled sequential release of trichloroacetic acid (TCA) and epidermal growth factor (EGF) on the oral soft tissue regeneration was determined.
Hydrophobically modified glycol chitosan (HGC) nano controlled system was developed for the sequential release of TCA and EGF, and the release pattern was identified. The HGC-based nano controlled release system was injected into the critical-sized defects created in beagles' palatal soft tissues. The palatal impression and its scanned body was obtained on various time points post-injection, and the volumetric amount of soft tissue regeneration was compared among the three groups: CON (natural regeneration control group), EXP1 (TCA-loaded nano controlled release system group), EXP2 (TCA and EGF individually loaded nano controlled release system). DNA microarray analysis was performed and various soft tissue regeneration parameters in histopathological specimens were measured.
TCA release was highest at Day 1 whereas EGF release was highest at Day 2 and remained high until Day 3. In the volumetric measurements of impression body scans, no significant difference in soft tissue regeneration between the three groups was shown in two-way ANOVA. However, in the one-way ANOVA at Day 14, EXP2 showed a significant increase in soft tissue regeneration compared to CON. High correlation was determined between the histopathological results of each group. DNA microarray showed up-regulation of various genes and related cell signaling pathways in EXP2 compared to CON.
HGC-based nano controlled release system for sequential release of TCA and EGF can promote regeneration of oral soft tissue defects.
本研究旨在探讨三氯乙酸（TCA）和表皮生长因子（EGF）纳米控制顺序释放对口腔软组织再生的影响。
研制了疏水性改性壳聚糖（HGC）纳米控制系统，用于 TCA 和 EGF 的顺序释放，并确定了释放模式。将基于 HGC 的纳米控释系统注入比格犬腭部软组织临界尺寸缺陷中。在注射后的各个时间点获取腭部印模及其扫描体，并比较三组之间的软组织再生体积量：CON（自然再生对照组）、EXP1（负载 TCA 的纳米控释系统组）、EXP2（单独负载 TCA 和 EGF 的纳米控释系统组）。进行 DNA 微阵列分析，并测量组织学标本中各种软组织再生参数。
TCA 释放量在第 1 天最高，EGF 释放量在第 2 天最高，并持续到第 3 天。在印模体扫描的体积测量中，三组之间在双向 ANOVA 中未显示出软组织再生的显著差异。然而，在第 14 天的单向 ANOVA 中，EXP2 与 CON 相比显示出软组织再生的显著增加。各组的组织病理学结果之间存在高度相关性。DNA 微阵列显示，与 CON 相比，EXP2 中各种基因和相关细胞信号通路的表达上调。
基于 HGC 的纳米控释系统用于 TCA 和 EGF 的顺序释放可以促进口腔软组织缺陷的再生。
