Cid A, Perona R, Serrano R
Instituto de Investigaciones Biomédicas del CSIC, Facultad de Medicina, Universidad Autónoma, Madrid, Spain.
Curr Genet. 1987;12(2):105-10. doi: 10.1007/BF00434664.
In order to probe the physiological role of the yeast plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.
为了探究酵母质膜ATP酶的生理作用，我们用半乳糖依赖性启动子取代了其基因的组成型启动子。当预先形成的ATP酶被稀释至正常水平的20%时，所得细胞在葡萄糖培养基上停止生长。ATP酶活性与细胞的质子外流和氨基酸转运之间存在相关性。很大一部分生长停滞的细胞看起来变大了，并且有几个含有细胞核的芽。
