Moss D M, Healy G R, Dickerson J W, Mathews H M
J Protozool. 1986 May;33(2):213-5. doi: 10.1111/j.1550-7408.1986.tb05592.x.
We used high-resolution polyacrylamide gradient gel electrophoresis (PGGE) to separate four babesial enzymes to aid in the identification of two Babesia microti isolates established in hamsters. The isolates were compared to two different hamsters passages of the "Gray" strain. All isoenzymes patterns from the two isolates and the "Gray" strain were similar except glucose phosphate isomerase (GPI) from one of the "Gray" strain passages. It showed a polymorphic GPI pattern as opposed to a monomorphic GPI pattern seen in the other "Gray" strain passage and the two isolates. The observed differences suggested that some population of B. microti are capable of having polymorphic GPI, that the "Gray" strain originally contained (and may still contain) a heterogeneous population of B. microti, and that the population possessing polymorphic GPI was selected over that with monomorphic GPI. This information was obtained by a PGGE method that eliminated hemoglobin from gels and allowed, for the first time, detection of babesial leucine amino peptidase (LAP) and isocitrate dehydrogenase (IDH). In addition, this method provided molecular weight estimations on babesial GPI, LAP, IDH, and glutamate dehydrogenase (GDH), and it proved useful in the identification and characterization of the B. microti isolates.
我们使用高分辨率聚丙烯酰胺梯度凝胶电泳（PGGE）来分离四种巴贝斯虫酶，以帮助鉴定在仓鼠体内建立的两种微小巴贝斯虫分离株。将这些分离株与“Gray”株的两种不同仓鼠传代进行比较。除了“Gray”株其中一个传代的葡萄糖磷酸异构酶（GPI）外，来自这两种分离株和“Gray”株的所有同工酶模式都相似。它显示出多态性GPI模式，而在另一个“Gray”株传代和这两种分离株中看到的是单态性GPI模式。观察到的差异表明，一些微小巴贝斯虫群体能够具有多态性GPI，“Gray”株最初包含（并且可能仍然包含）微小巴贝斯虫的异质群体，并且具有多态性GPI的群体比具有单态性GPI的群体更具优势。这些信息是通过一种PGGE方法获得的，该方法从凝胶中去除了血红蛋白，并首次检测到巴贝斯虫亮氨酸氨基肽酶（LAP）和异柠檬酸脱氢酶（IDH）。此外，该方法还提供了对巴贝斯虫GPI、LAP、IDH和谷氨酸脱氢酶（GDH）的分子量估计，并且在微小巴贝斯虫分离株的鉴定和表征中证明是有用的。
