Moura H, Wallace S, Visvesvara G S
Parasitic Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Protozool. 1992 Sep-Oct;39(5):573-83. doi: 10.1111/j.1550-7408.1992.tb04853.x.
Two strains of Acanthamoeba isolated from human brain tissue and a strain of Acanthamoeba isolated from a fish were compared with 10 species of Acanthamoeba belonging to groups 1, 2 and 3 based on their isoenzyme profiles and antigenic characteristics. A total of 12 enzymes were studied. The isoenzymes and antigens were electrophoretically separated on polyacrylamide gradient gels, and the patterns obtained were compared after appropriate staining for particular enzymes and reactivities with homologous and heterologous rabbit anti-Acanthamoeba antisera. One of the human strains (CDC:1283:V013) was identified as A. healyi n. sp. because of its unique isoenzyme profiles for 11 of the 12 enzymes tested. The other human isolate was reidentified as A. culbertsoni because its isoenzyme profiles for 10 of 12 enzymes resembled those of A. culbertsoni, Lilly A-1 strain. Since the isoenzyme profiles and the antigenic patterns of the fish isolate as well were remarkably similar to those of A. royreba, it was considered as a strain of A. royreba. Polyacrylamide gradient gel electrophoresis appears to be a powerful technique for the study of isoenzymes and antigens of Acanthamoeba.
基于同工酶谱和抗原特性，将从人脑组织分离出的两株棘阿米巴和从鱼体分离出的一株棘阿米巴与属于第1、2和3组的10种棘阿米巴进行了比较。共研究了12种酶。同工酶和抗原在聚丙烯酰胺梯度凝胶上进行电泳分离，在对特定酶进行适当染色并与同源和异源兔抗棘阿米巴抗血清反应后，比较所得图谱。其中一株人源菌株（CDC:1283:V013）被鉴定为新种希氏棘阿米巴，因为在所测试的12种酶中有11种具有独特的同工酶谱。另一株人源分离株被重新鉴定为卡氏棘阿米巴，因为其12种酶中有10种的同工酶谱与卡氏棘阿米巴Lilly A-1菌株相似。由于鱼源分离株的同工酶谱和抗原模式也与罗氏棘阿米巴非常相似，因此被认为是罗氏棘阿米巴的一个菌株。聚丙烯酰胺梯度凝胶电泳似乎是研究棘阿米巴同工酶和抗原的有力技术。
