Center for Strategic Research Project, Organization for Research Promotion, University of the Ryukyus, Okinawa, Japan.
Department of Integrative Genomics, Tohoku Medial Megabank Organization, Tohoku University, Miyagi, Japan.
Mol Biol Evol. 2018 Jun 1;35(6):1553-1555. doi: 10.1093/molbev/msy074.
Fish mitochondrial genome (mitogenome) data form a fundamental basis for revealing vertebrate evolution and hydrosphere ecology. Here, we report recent functional updates of MitoFish, which is a database of fish mitogenomes with a precise annotation pipeline MitoAnnotator. Most importantly, we describe implementation of MiFish pipeline for metabarcoding analysis of fish mitochondrial environmental DNA, which is a fast-emerging and powerful technology in fish studies. MitoFish, MitoAnnotator, and MiFish pipeline constitute a key platform for studies of fish evolution, ecology, and conservation, and are freely available at http://mitofish.aori.u-tokyo.ac.jp/ (last accessed April 7th, 2018).
鱼类线粒体基因组（mitogenome）数据为揭示脊椎动物进化和水圈生态学提供了基础。在这里，我们报告了 MitoFish 的最新功能更新，MitoFish 是一个带有精确注释管道 MitoAnnotator 的鱼类线粒体基因组数据库。最重要的是，我们描述了 MiFish 管道在鱼类线粒体环境 DNA 宏条形码分析中的实现，这是鱼类研究中一种快速发展且强大的技术。MitoFish、MitoAnnotator 和 MiFish 管道构成了鱼类进化、生态学和保护研究的关键平台，可在 http://mitofish.aori.u-tokyo.ac.jp/（最后访问日期为 2018 年 4 月 7 日）免费获取。
