Department of Clinical Laboratory, The University of Tokyo Hospital.
Department of Lipidomics, Graduate School of Medicine, The University of Tokyo.
J Occup Health. 2018 Jul 25;60(4):324-332. doi: 10.1539/joh.2018-0027-FS. Epub 2018 Apr 18.
Rotating shift work has been reported to increase the risk of cardiovascular diseases. Vascular endothelial dysfunction and platelet activation are among the leading causes of thrombus formation in patients with myocardial infarction or stroke. Endothelial function has been shown to be impaired immediately after night-shift work; however, it is not known whether platelets are also activated. The aim of this study was to investigate the acute impact of night-shift work on platelet function.
This observational study included 11 healthy medical staff members (seven women, median age 32 years). We examined each subject's platelet aggregation rates and the serum concentrations of eicosanoid mediators after night-shift work and on day-shift work without preceding night-shift work (baseline).
Platelet aggregation did not differ from baseline levels after night-shift work. However, serum cyclooxygenase (COX)-metabolized eicosanoid mediators, particularly thromboxane (Tx) B (a stable metabolite of TxA and the most important marker of platelet activation), were significantly higher after the night-shift than at baseline (median 65.3 vs 180.4 ng/ml).
Although platelet aggregation did not increase, there was an increase in serum COX-metabolized eicosanoid mediators such as TxB in healthy medical staff after night-shift work. This platelet hypersensitivity may be one of the mechanisms underlying the significant association between night-shift work and adverse cardiovascular outcomes.
轮班工作已被报道会增加心血管疾病的风险。血管内皮功能障碍和血小板激活是心肌梗死或中风患者血栓形成的主要原因之一。研究表明，夜班工作后内皮功能立即受损；然而，目前尚不清楚血小板是否也被激活。本研究旨在探讨夜班工作对血小板功能的急性影响。
本观察性研究纳入了 11 名健康的医务人员（7 名女性，中位年龄 32 岁）。我们在夜班工作后和没有前夜班工作的白班工作时（基线）检查了每个受试者的血小板聚集率和环氧合酶（COX）代谢的类花生酸介质的血清浓度。
血小板聚集在夜班工作后与基线水平无差异。然而，血清环氧化酶（COX）代谢的类花生酸介质，特别是血栓素（Tx）B（TxA 的稳定代谢物，也是血小板激活的最重要标志物），在夜班后明显高于基线（中位数 65.3 对 180.4ng/ml）。
尽管血小板聚集没有增加，但在夜班工作后，健康医务人员的血清 COX 代谢的类花生酸介质如 TxB 增加。这种血小板高敏感性可能是夜班工作与不良心血管结局之间显著关联的机制之一。
