Shepherd Frances K, Herrera-Ibata Diana Maria, Porter Elizabeth, Homwong Nitipong, Hesse Richard, Bai Jianfa, Marthaler Douglas G
Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
Pathogens. 2018 Apr 18;7(2):44. doi: 10.3390/pathogens7020044.
Rotaviruses (RVs) are a major etiological agent of acute viral gastroenteritis in humans and young animals, with rotavirus B (RVB) often detected in suckling and weaned pigs. Group A rotavirus classification is currently based on the two outer capsid proteins, VP7 and VP4, and the middle layer protein, VP6. Using RVB strains generated in this study and reference sequences from GenBank, pairwise identity frequency graphs and phylogenetic trees were constructed for the eleven gene segments of RVB to estimate the nucleotide identity cutoff values for different genotypes and determine the genotype diversity per gene segment. Phylogenetic analysis of VP7, VP4, VP6, VP1–VP3, and NSP1–NSP5 identified 26G, 5P, 13I, 5R, 5C, 5M, 8A, 10N, 6T, 4E, and 7H genotypes, respectively. The analysis supports the previously proposed cutoff values for the VP7, VP6, NSP1, and NSP3 gene segments (80%, 81%, 76% and 78%, respectively) and suggests new cutoff values for the VP4, VP1, VP2, VP3, NSP2, NSP4, and NSP5 (80%, 78%, 79%, 77% 83%, 76%, and 79%, respectively). Reassortment events were detected between the porcine RVB strains from our study. This research describes the genome constellations for the complete genome of Group B rotaviruses in different host species.
轮状病毒（RVs）是人类和幼龄动物急性病毒性胃肠炎的主要病原体，B组轮状病毒（RVB）常在哺乳仔猪和断奶仔猪中被检测到。目前，A组轮状病毒的分类是基于两种外衣壳蛋白VP7和VP4以及中层蛋白VP6。利用本研究中产生的RVB毒株和来自GenBank的参考序列，构建了RVB 11个基因片段的成对同一性频率图和系统发育树，以估计不同基因型的核苷酸同一性截止值，并确定每个基因片段的基因型多样性。对VP7、VP4、VP6、VP1-VP3和NSP1-NSP5的系统发育分析分别鉴定出26G、5P、13I、5R、5C、5M、8A、10N、6T、4E和7H基因型。该分析支持先前提出的VP7、VP6、NSP1和NSP3基因片段的截止值（分别为80%、81%、76%和78%），并提出了VP4、VP1、VP2、VP3、NSP2、NSP4和NSP5的新截止值（分别为80%、78%、79%、77%、83%、76%和79%）。在本研究的猪RVB毒株之间检测到了重配事件。本研究描述了不同宿主物种中B组轮状病毒全基因组的基因组构成。
