Dote Joël W, Banga Mingo Virginie, Fandema Jean, Gody Jean C, Mwenda Jason M, Esona Mathew D, Bowen Michael D, Komas Narcisse Patrice J, Gouandjika-Vasilache Ionela, Waku-Kouomou Diane
Institut Pasteur de Bangui, BP 923, Bangui, Central African Republic.
Pediatric University Hospital Center of Bangui, Bangui, Central African Republic.
BMC Infect Dis. 2025 May 8;25(1):681. doi: 10.1186/s12879-025-11057-4.
Rotavirus gastroenteritis is one of major causes of death in infants, particularly in sub-Saharan Africa. In the Central African Republic (CAR), sentinel surveillance of rotavirus gastroenteritis was established in 2011. In this study, we assessed the burden of rotavirus gastroenteritis and identified rotavirus strains circulating in CAR during 2011-2021.
Stool samples were collected from < 5-year-old children with diarrhoea according to WHO criteria, at the sentinel site in Bangui, CAR. Samples were screened for group A rotavirus antigen by EIA. RNA was extracted from all EIA-positive samples which were subjected to genotyping using a semi nested RT-PCR assay.
From 2011 to 2021, 1855 stool samples were collected and 854 (46.0%) were positive for rotavirus by EIA. Genotypes were obtained from 77.3% (660/854) EIA positive samples. Of these 660 samples, genotypes funds were: G1 (35.4%) and G2 (26.6%) for VP7, and P[6] (42.7%) and P[8] (35.6%) for the VP4 gene. The most frequent genotype combinations were G1P[8], 19.3% and G1P[6], 15.0%.
This study reports the prevalence of rotavirus genotypes that circulated for ten years, providing a pre-vaccine baseline data genotype estimate for rotavirus gastroenteritis sentinel surveillance in the Central African Republic.
Not applicable.
轮状病毒肠胃炎是婴儿死亡的主要原因之一，在撒哈拉以南非洲地区尤为如此。在中非共和国，2011年建立了轮状病毒肠胃炎哨点监测。在本研究中，我们评估了轮状病毒肠胃炎的负担，并确定了2011年至2021年期间在中非共和国传播的轮状病毒株。
根据世界卫生组织标准，在中非共和国班吉的哨点采集5岁以下腹泻儿童的粪便样本。通过酶免疫测定法筛选样本中的A组轮状病毒抗原。从所有酶免疫测定法呈阳性的样本中提取RNA，并使用半巢式逆转录聚合酶链反应测定法进行基因分型。
2011年至2021年期间，共采集了1855份粪便样本，其中854份（46.0%）通过酶免疫测定法检测轮状病毒呈阳性。从77.3%（660/854）的酶免疫测定法阳性样本中获得了基因型。在这660份样本中，检测到的基因型有：VP7基因的G1（35.4%）和G2（26.6%），以及VP4基因的P[6]（42.7%）和P[8]（35.6%）。最常见的基因型组合是G1P[8]，占19.3%，以及G1P[6]，占15.0%。
本研究报告了十年间传播的轮状病毒基因型的流行情况，为中非共和国轮状病毒肠胃炎哨点监测提供了疫苗接种前基因型估计的基线数据。
不适用。
