帕金森病血浆样本中异常循环 miRNA 的鉴定。
Identification of aberrant circulating miRNAs in Parkinson's disease plasma samples.
机构信息
Department of NeurologyTianjin Huan Hu Hospital Jinnan District, Tianjin China.
Tianjin Key Laboratory of Cerebrovascular and Neurodegenerative Diseases Tianjin China.
出版信息
Brain Behav. 2018 Feb 19;8(4):e00941. doi: 10.1002/brb3.941. eCollection 2018 Apr.
OBJECTIVE
To detect the aberrant expression of circulating miRNAs and explore the potential early diagnostic biomarkers in patients with Parkinson's disease (PD).
METHODS
Plasma samples were collected from 25 treatment-naïve PD-diagnosed patients and 25 healthy controls followed by a real-time PCR-based miRNA screening analysis of neuron disease-related miRNAs.
RESULTS
A subset of miRNAs with aberrant expression levels in the plasma of PD-diagnosed patients were identified including upregulation of miR-27a and downregulation of let-7a, let-7f, miR-142-3p, and miR-222 with the AUC values more than 0.8 derived from the receiver operating characteristic curves.
CONCLUSIONS
The high sensitivity and specificity of the circulating miRNAs may enable early diagnosis of PD. The study provides a group of novel miRNA candidates for detecting PD.
目的
检测循环 miRNA 的异常表达，探索帕金森病（PD）患者潜在的早期诊断生物标志物。
方法
收集 25 例未经治疗的 PD 确诊患者和 25 例健康对照者的血浆样本，随后进行基于实时 PCR 的神经元疾病相关 miRNA 的 miRNA 筛选分析。
结果
鉴定出一组在 PD 确诊患者血浆中表达异常的 miRNAs，包括 miR-27a 的上调和 let-7a、let-7f、miR-142-3p 和 miR-222 的下调，其 AUC 值大于来自受试者工作特征曲线的 0.8。
结论
循环 miRNA 的高灵敏度和特异性可能使 PD 能够早期诊断。该研究为检测 PD 提供了一组新的 miRNA 候选物。
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