Mohammadi Farhad, Mohammadi Samani Soliman, Tanideh Nader, Ahmadi Fatemeh
Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Center for Nanotechnology in Drug Delivery, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Adv Pharm Bull. 2018 Mar;8(1):11-19. doi: 10.15171/apb.2018.002. Epub 2018 Mar 18.
Cartilage regeneration by using polymeric scaffolds is a new option for treatment of osteoarthritis. A good scaffold for tissue engineering should copy the characteristics of natural extracellular matrix. The purpose of this study was to make a dosage form with proper reliability and stability for cartilage repair. Hybrid scaffolds containing different ratios of hyaluronic acid (HA) and collagen were prepared and loaded with prednisolone as anti-inflammatory agent. Two different dosage forms (lyophilized implantable disk and thermo-sensitive gels) were examined. A scaffold of cross-linked HA was used as control. Different characterization tests were considered including differential scanning calorimetry (DSC), scanning electron microscopy, mechanical evaluations, and drug release. The physical and chemical performance of hybrid-scaffolds was better than HA scaffold. Increasing the concentration of HA and collagen improved the physical and chemical characteristics. Regarding the mechanical properties of the hybrid scaffold, the pore size was 20-200µm, compressive modulus was 54.77±0.31 kPa, more than 1200% water uptake was observed after 4 days, gelation temperature was 32±0.16°C, gelation time was 2.4±0.1 min, and drug release was controlled for 5 days by Higuchi release kinetic model. It seems that this porous hybrid scaffold could be a suitable choice in cartilage regeneration as well as a controlled-release system for delivery of prednisolone in osteoarthritis.
使用聚合物支架进行软骨再生是治疗骨关节炎的一种新选择。用于组织工程的良好支架应模仿天然细胞外基质的特性。本研究的目的是制备一种具有适当可靠性和稳定性的剂型用于软骨修复。制备了含有不同比例透明质酸（HA）和胶原蛋白的混合支架，并负载泼尼松龙作为抗炎剂。研究了两种不同的剂型（冻干可植入盘和热敏凝胶）。使用交联HA支架作为对照。考虑了不同的表征测试，包括差示扫描量热法（DSC）、扫描电子显微镜、力学评估和药物释放。混合支架的物理和化学性能优于HA支架。增加HA和胶原蛋白的浓度改善了物理和化学特性。关于混合支架的力学性能，孔径为20 - 200µm，压缩模量为54.77±0.31 kPa，4天后吸水率超过1200%，凝胶化温度为32±0.16°C，凝胶化时间为2.4±0.1分钟，药物释放通过Higuchi释放动力学模型控制5天。看来这种多孔混合支架可能是软骨再生的合适选择，也是骨关节炎中泼尼松龙递送的控释系统。
