Quiram Gina, Montagner Francisco, Palmer Kelli L, Stefan Mihaela C, Washington Katherine E, Rodrigues Danieli C
Department of Bioengineering, University of Texas at Dallas, 800 W Campbell, Richardson, TX 75080, USA.
Department of Conservative Dentistry, Dental School, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2492, 90460-001 Porto Alegre, Brazil.
J Funct Biomater. 2018 Apr 17;9(2):29. doi: 10.3390/jfb9020029.
Persistence of microorganisms in dentinal tubules after root canal chemo-mechanical preparation has been well documented. The complex anatomy of the root canal and dentinal buffering ability make delivery of antimicrobial agents difficult. This work explores the use of a novel trilayered nanoparticle (TNP) drug delivery system that encapsulates chlorhexidine digluconate, which is aimed at improving the disinfection of the root canal system. Chlorhexidine digluconate was encapsulated inside polymeric self-assembled TNPs. These were self-assembled through water-in-oil emulsion from poly(ethylene glycol)--poly(lactic acid) (PEG--PLA), a di-block copolymer, with one hydrophilic segment and another hydrophobic. The resulting TNPs were physicochemically characterized and their antimicrobial effectiveness was evaluated against using a broth inhibition method. The hydrophilic interior of the TNPs successfully entrapped chlorhexidine digluconate. The resulting TNPs had particle size ranging from 140–295 nm, with adequate encapsulation efficiency, and maintained inhibition of bacteria over 21 days. The delivery of antibacterial irrigants throughout the dentinal matrix by employing the TNP system described in this work may be an effective alternative to improve root canal disinfection.
根管化学机械预备后微生物在牙本质小管中的持续存在已得到充分证明。根管复杂的解剖结构和牙本质的缓冲能力使得抗菌剂的输送变得困难。这项研究探索了一种新型的三层纳米颗粒（TNP）药物递送系统，该系统包裹葡萄糖酸氯己定，旨在改善根管系统的消毒效果。葡萄糖酸氯己定被包裹在聚合物自组装的TNP内部。这些纳米颗粒是通过油包水乳液由聚（乙二醇）-聚（乳酸）（PEG-PLA）自组装而成的，PEG-PLA是一种二嵌段共聚物，有一个亲水链段和另一个疏水链段。对所得的TNP进行了物理化学表征，并使用肉汤抑制法评估了它们对细菌的抗菌效果。TNP的亲水性内部成功地包裹了葡萄糖酸氯己定。所得的TNP粒径范围为140-295nm，具有足够的包封效率，并在超过21天的时间内保持对细菌的抑制作用。通过采用本文所述的TNP系统在整个牙本质基质中递送抗菌冲洗液可能是改善根管消毒的一种有效替代方法。
