Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, People's Republic of China.
Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, People's Republic of China.
J Nanobiotechnology. 2018 Apr 19;16(1):42. doi: 10.1186/s12951-018-0367-9.
AgS has the characteristics of conventional quantum dot such as broad excitation spectrum, narrow emission spectrum, long fluorescence lifetime, strong anti-bleaching ability, and other optical properties. Moreover, since its fluorescence emission is located in the NIR-II region, has stronger penetrating ability for tissue. AgS quantum dot has strong absorption during the visible and NIR regions, it has good photothermal and photoacoustic response under certain wavelength excitation.
200 nm aqueous probe AgS@DSPE-PEG-FA (AgS@DP-FA) with good dispersibility and stability was prepared by coating hydrophobic AgS with the mixture of folic acid (FA) modified DSPE-PEG (DP) and other polymers, it was found the probe had good fluorescent, photoacoustic and photothermal responses, and a low cell cytotoxicity at 50 μg/mL Ag concentration. Blood biochemical analysis, liver enzyme and tissue histopathological test showed that no significant influence was observed on blood and organs within 15 days after injection of the probe. In vivo and in vitro fluorescence and photoacoustic imaging of the probe further demonstrated that the AgS@DP-FA probe had good active targeting ability for tumor. In vivo and in vitro photothermal therapy experiments confirmed that the probe also had good ability of killing tumor by photothermal.
AgS@DP-FA was a safe, integrated diagnosis and treatment probe with multi-mode imaging, photothermal therapy and active targeting ability, which had a great application prospect in the early diagnosis and treatment of tumor.
AgS 具有常规量子点的特点，如宽激发光谱、窄发射光谱、长荧光寿命、强抗漂白能力和其他光学性质。此外，由于其荧光发射位于近红外二区（NIR-II 区），具有更强的组织穿透能力。AgS 量子点在可见光和近红外区有很强的吸收，在一定波长激发下具有良好的光热和光声响应。
通过将疏水性 AgS 包裹在叶酸（FA）修饰的二硬脂酰基磷脂酰乙醇胺-聚乙二醇（DSPE-PEG）和其他聚合物的混合物中，制备出了具有良好分散性和稳定性的 200nm 水性探针 AgS@DSPE-PEG-FA（AgS@DP-FA），发现该探针具有良好的荧光、光声和光热响应，在 50μg/mL Ag 浓度下细胞毒性低。血液生化分析、肝酶和组织组织病理学试验表明，在注射探针后 15 天内，血液和器官未观察到明显影响。探针的体内和体外荧光和光声成像进一步证明了 AgS@DP-FA 探针对肿瘤具有良好的主动靶向能力。体内和体外光热治疗实验证实，该探针还具有通过光热杀伤肿瘤的良好能力。
AgS@DP-FA 是一种安全、集成的多模式成像、光热治疗和主动靶向治疗探针，在肿瘤的早期诊断和治疗中有很大的应用前景。
