Analgesic and Anti-Inflammatory Effects of 80% Methanol Extract of (Burm.f.) Iwarsson Leaves in Rodent Models.

BACKGROUND

Pain and inflammation are the major health problems commonly treated with traditional remedies mainly using medicinal plants. is one of such medicinal plants used in folkloric medicine of Ethiopia. However, the plant has not been scientifically evaluated. The aim of this study was to evaluate analgesic and anti-inflammatory effects of the 80% methanol leaves extract of using rodent models.

METHOD

The central and peripheral analgesic effect of the extract at 100, 200, and 400 mg/kg dose levels was evaluated using hot plate and acetic acid induced writhing rodent models, whereas carrageenan induced paw edema and cotton pellet granuloma methods were used to screen anti-inflammatory effect of the extract at the same dose levels. Acute toxicity test was also done. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test and < 0.05 was considered significant.

RESULTS

The extract did not produce mortality up to 2000 mg/kg. All tested doses of the extract showed significant analgesic effect with maximum latency response of 62.8% and inhibition of acetic acid induced writhing. Maximum anti-inflammatory effect was recorded at 6 h after induction, with 75.88% reduction in carrageenan induced paw edema. Moreover, all tested doses of extract significantly inhibited the formation of inflammatory exudates and granuloma formation ( < 0.001).

CONCLUSION

The study indicated that the extract was safe in mice and it has both analgesic and anti-inflammatory effect in rodent models.