Department of Pharmaceutical Biology, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Toxins (Basel). 2019 Jul 12;11(7):406. doi: 10.3390/toxins11070406.
() extracts exhibit antimicrobial activity due to the complex alkaloid composition. The aim of the research was to evaluate the antimicrobial potential of extracts from wild plants and in vitro cultures, as well as seven major individual alkaloids. Plant material derived from different natural habitats and in vitro cultures was used for the phytochemical analysis and antimicrobial tests. The composition of alkaloids was analyzed using chromatographic techniques (HPLC with DAD detection). The results have shown that roots contained higher number and amounts of alkaloids in comparison to aerial parts. All tested plant extracts manifested antimicrobial activity, related to different chemical structures of the alkaloids. Root extract used at 31.25-62.5 mg/L strongly reduced bacterial biomass. From the seven individually tested alkaloids, chelerythrine was the most effective against (MIC at 1.9 mg/L), while sanguinarine against (MIC at 1.9 mg/L). Strong antifungal activity was observed against when chelerythrine, chelidonine, and aerial parts extract were used. The experiments with plant extracts, individually tested alkaloids, and variable combinations of the latter allowed for a deeper insight into the potential mechanisms affecting the activity of this group of compounds.
（）提取物由于其复杂的生物碱成分而具有抗菌活性。本研究的目的是评估野生植物和体外培养物提取物以及七种主要单体生物碱的抗菌潜力。用于植物化学分析和抗菌测试的植物材料来自不同的自然生境和体外培养物。使用色谱技术（带有 DAD 检测的 HPLC）分析生物碱的组成。结果表明，与地上部分相比，根部的生物碱数量和含量更高。所有测试的植物提取物均表现出抗菌活性，这与生物碱的不同化学结构有关。在 31.25-62.5mg/L 浓度下，根提取物强烈降低了细菌生物量。在所测试的七种单体生物碱中，白屈菜红碱对 （MIC 为 1.9mg/L）最有效，而血根碱对 （MIC 为 1.9mg/L）最有效。当使用白屈菜红碱、白屈菜碱和地上部分提取物时，对 表现出强烈的抗真菌活性。使用植物提取物、单体生物碱以及后者的可变组合进行的实验使我们能够更深入地了解影响这组化合物活性的潜在机制。
