基于相干的细胞成像的扩展焦深
Extended depth of focus for coherence-based cellular imaging.
作者信息
Yin Biwei, Hyun Chulho, Gardecki Joseph A, Tearney Guillermo J
机构信息
Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, 55 Fruit Street, Boston, Massachusetts 02114, USA.
Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
出版信息
Optica. 2017 Aug;4(8):959-965. doi: 10.1364/OPTICA.4.000959. Epub 2017 Aug 9.
Improving lateral resolution for cross-sectional optical coherence tomography (OCT) imaging is difficult due to the rapid divergence of light once it is focused to a small spot. To overcome this obstacle, we introduce a fiber optics system that generates a coaxially focused multimode (CAFM) beam for depth of focus (DOF) extension. We fabricated a CAFM beam OCT probe and show that the DOF is more than fivefold that of a conventional Gaussian beam, enabling cross-sectional imaging of biological tissues with clearly resolved cellular and subcellular structures over more than a 400 μm depth range. The compact and straightforward design and high-resolution extended DOF imaging capabilities of this technique suggests that it will be very useful for endoscopic cross-sectional imaging of human internal organs .
由于光一旦聚焦到一个小光斑就会迅速发散，因此提高用于横截面光学相干断层扫描（OCT）成像的横向分辨率很困难。为了克服这一障碍，我们引入了一种光纤系统，该系统可产生用于扩展焦深（DOF）的同轴聚焦多模（CAFM）光束。我们制作了一个CAFM光束OCT探头，并表明其焦深是传统高斯光束的五倍以上，能够在超过400μm的深度范围内对生物组织进行横截面成像，清晰分辨细胞和亚细胞结构。该技术紧凑、简单的设计以及高分辨率扩展焦深成像能力表明，它将对人体内部器官的内窥镜横截面成像非常有用。
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